来自海黄瓜液的富科伊丹:结构分析,物理化学性质和抗Helicobacter pylori潜在的潜力
Junhan Cao1, Ling Qin2, Ming Liu3
1College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; Qingdao Key Laboratory of Marine Natural Products Research and Development Laboratory, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China.
International journal of biological macromolecules
|March 2, 2025
概括
海黄瓜富科伊丹 (Fuc-SC) 通过破坏细菌结构和减少尿素酶活性,有效地抑制了Helicobacter pylori (H. pylori) 的生长和粘附. 这种天然化合物显示出作为一种新型治疗剂的希望,用于治疗H. pylori感染.
科学领域:
- 海洋天然产品 海洋天然产品
- 微生物学 微生物学
- 生物化学 生物化学
- 胃肠病学 胃肠病学
背景情况:
- 杆菌 (H. pylori) 感染是胃炎,胃潰瘍和胃癌的主要原因.
- 增加抗生素耐药性和细菌持久性需要开发替代治疗策略.
- 富科伊丹是棕藻中的硫酸多糖,已经证明了各种生物活性,但其来自其他海洋来源的潜力仍未得到充分探索.
研究的目的:
- 研究从海黄瓜液 (Fuc-SC) 中提取的fucoidan对H.pylori的抑制作用.
- 描述Fuc-SC.的结构和物理化学特性.
- 在体外评估Fuc-SC的抗氧化和抗H. pylori活性.
主要方法:
- 使用分子量测定,糖和硫酸盐含量分析以及链接分析,对Fuc-SC的结构性表征.
- 物理化学性质评估包括原子力显微镜 (AFM),扫描电子显微镜 (SEM),热稳定性和性行为.
- 在体外抗氧化剂测试 (ABTS,DPPH,总抗氧化能力) 和针对H. pylori SS1的抗菌测试,包括抑制区,尿酶活性,细胞粘附和生物膜形成抑制.
主要成果:
- 鉴定Fuc-SC是一种分支硫酸富可伊丹 (6.75kDa),具有独特的硫化模式和不规则的线性链形状.
- 与拉米纳里亚日本菌衍生的福柯伊丹 (Fuc-LJ) 相比,Fuc-SC在体外表现出更强的抗氧化活性和显著的H. pylori生长抑制.
- 通过抑制尿酶活性,Fuc-SC有效地降低了H. pylori的粘附性,保护胃细胞免受氧化应激,并破坏了细菌结构和生物膜形成.
结论:
- 来自海的富科伊丹 (Fuc-SC) 具有显著的抗氧化和抗H. pylori特性,包括抑制尿酶活性和生物膜形成.
- Fuc-SC的独特结构特征有助于其强大的生物活性.
- Fuc-SC具有相当大的发展潜力,可用于食补充剂和特殊医疗食品,用于管理与H. pylori相关的胃肠道疾病.
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