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对差异化转录组表达的分析表明,ISG15通过促进猪中位膜中的血管生成,为胚胎发育提供支持

Jianyu Gan¹, Yongzhong Wang¹, Fan Zhang¹

¹State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.

International journal of biological macromolecules

|March 2, 2025

概括

研究中介质层的研究.

科学领域:

生殖生物学生殖生物学
血管生物学血管生物学
基因组学就是基因组学.

背景情况:

中子宫通过其血管系统作为子宫内膜和外部环境之间营养和物质交换的关键接口.
了解介质膜的转录格局对于阐明它在成功胚胎植入中的作用至关重要.

研究的目的:

在雌性周期和怀孕期间创建子宫内膜和相邻子宫内膜的转录图谱.
在植入过程中识别关键的基因和涉及到介质膜功能的途径.

主要方法:

在雌性周期和怀孕的第12天对子宫内膜和子宫内膜样本进行转录组分析.
免疫组织化学用于定位血管内皮细胞内的核心基因表达.
在体外测试以评估ISG15 (干扰素α和马信号传递) 对细胞过程的功能影响.

主要成果:

在子宫内膜和子宫内膜中观察到与干扰素信号传递相关的基因 (例如ISG15) 的显著丰富.
核心干扰素相关的基因被定位到两种组织中的血管内皮细胞中.
下调ISG15促进了细胞增殖,迁移和管形成在体外.

结论:

在子宫内膜和子宫内膜中对干扰素反应基因的降低调节有助于血管生成.
中子宫通过调节血管生成,在怀孕中发挥着重要的支持作用.

关键词:

在ISG15中,我们可以看到ISG15.对干扰素的反应测量器 (Mesometrium) 是一种测量器.猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪转录组转录组就是一个转录组.

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