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基于生物信息学结合单细胞RNA-seq和多重机器学习分析来识别心血管化中的潜在生物标志物

Bingchen Guo¹, Si Shi², Jie Xiong¹

¹Harbin Medical University, Harbin, China; Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, Harbin 150000, China.

Cellular signalling

|March 2, 2025

概括

这项研究确定ITGAX和MYD88是心血管化的关键生物标志物,揭示了它们在化组织中的上调调节和与免疫反应的关联. 这些发现表明血管化的潜在诊断和治疗点.

科学领域:

心血管生物学心血管生物学
分子遗传学分子遗传学
免疫学免疫学免疫学

背景情况:

血管化的分子和遗传基础尚未完全理解.
这项研究的重点是确定巨细胞内化标志物相关基因表达的差异.

研究的目的:

为了确定关键的基因和参与血管化的途径.
探索心血管化的潜在诊断和治疗点.

主要方法:

对基因表达数据集 (GSE104140,GSE235995) 的分析,以找到差异表达的基因.
利用基因本体学,KEGG,WGCNA,GSEA,PPI网络和机器学习进行功能和枢纽基因分析.
使用单细胞RNA测序,门德尔随机化,ELISA,免疫组织化学和血管内超声波验证的结果.

主要成果:

确定ITGAX和MYD88是心血管化的重要诊断生物标志物.
在化样本中观察到ITGAX和MYD88的显著上调,与免疫过程有关.
通过单细胞RNA测序在免疫细胞中证实了这些生物标志物的高表达,并通过ELISA和免疫组织化学验证.

结论:

ITGAX和MYD88是心血管化的关键生物标志物,与免疫反应有关.
网氨酸与ITGAX和MYD88的相互作用被证明,表明了潜在的治疗途径.
这项研究为血管化机制提供了新的见解,并确定了介入的有希望的目标.

关键词:

生物标志物生物标志物化是一种化过程.不同表达的基因.枢纽基因枢纽基因枢纽基因巨细胞是一个巨细胞.

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