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三种类型的17β-hydroxysteroid脱酶参与了日本鱼卵巢类固醇生成

Hiroshi Suzuki¹, Risa Ito², Yuichi Ozaki²

¹Fisheries Technology Institute, Shibushi Field Station, Japan Fisheries Research and Education Agency, 205 Natsui, Shibushi, Shibushi, Kagoshima 899-7101, Japan.

General and comparative endocrinology

|March 2, 2025

概括

这项研究确定了关键酶,17β-hydroxysteroid dehydrogenases (Hsd17bs),参与了日本鱼卵巢中的雌激醇-17β (E2) 合成. 在卵巢发育过程中,HSD17b1对E2的产生至关重要.

科学领域:

内分泌学在内分泌学.
分子生物学分子生物学
鱼类的繁殖鱼类的繁殖

背景情况:

雌激醇-17β (E2) 对于鱼类体育生成至关重要,由类固醇酶合成.
17β-hydroxysteroid脱酶 (Hsd17bs) 对于性别类固醇激素的合成至关重要.
虽然已知HSD17b1在远程生物中对E2合成起作用,但其他HSD17b类型的作用尚不太清楚.

研究的目的:

从日本鱼中分离和描述HSD17b7.
调查HSD17b1,HSD17b7和HSD17b12a在卵巢发育期间E2合成中的作用.
为了比较这些Hsd17bs的酶性质和表达模式.

主要方法:

日本鱼的cDNA分离和测序 Hsd17b7.7.
重组蛋白表达和酶活性测定 (A4到T,E1到E2转换).
定量实时PCR用于组织分布和基因表达分析.

主要成果:

日本鱼Hsd17b7cDNA编码了一种蛋白质,具有保留的短链脱酶特征.
重组Hsd17b7和Hsd17b12a表现出将androstenedione转化为和雌激素转化为E2.2的能力.
在未成熟的鱼卵巢中检测到HSD17b1,HSD17b7和HSD17b12amRNA,卵巢发育过程中HSD17b1的表达显著增加.

结论:

Hsd17b1,Hsd17b7和Hsd17b12a与日本鱼的卵巢E2合成有关.
在 oogenesis 过程中,HSD17b1 在 E2 生产中起着主导作用.
需要进一步研究HSD17b7和HSD17b12a在鱼繁殖中的特定功能.

关键词:

17β-基类固醇脱酶1型 17β-基类固醇脱酶12a型 17β-基类固醇脱酶7型 Estradiol-17ββ的使用情况日本鱼日本鱼卵巢卵巢卵巢

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