环境过渡导航表型切换,影响致病性Morganella morganii的毒性和多药耐药性
Vikash Kumar1, Basanta Kumar Das1, Suvra Roy1
1Aquatic Environmental Biotechnology (AEB) Division, ICAR-Central Inland Fisheries Research Institute (CIFRI), Barrackpore, 700120, India.
Microbial pathogenesis
|March 2, 2025
概括
震动条件影响多药耐药细菌摩根氏菌. 非震动条件促进生物膜的形成和毒性,而震动可以减少这些特征,提供控制策略.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 环境微生物学 环境微生物学
背景情况:
- 细菌的表型转换对于生存,社区形成,抗压力和毒性至关重要.
- 摩根氏菌 (Morganella morganii) 是一种具有多种耐药性和致病性的细菌.
- 环境因素,如流体剪切,可以显著影响细菌的行为和毒性.
研究的目的:
- 调查震动条件对摩根氏菌 (Morganella morganii) 中的表型切换的作用.
- 分析震动对生物膜形成,蛋白质分泌,抗生素耐药性和毒性的影响.
- 通过操纵流体剪切来探索在水产养殖中控制M. morganii的潜力.
主要方法:
- 在震动和非震动条件下培养M. morganii在体外.
- 评估生物膜形成,蛋白质分泌和多重抗生素耐药性 (MAR) 值.
- 评估体外和体外毒性因素,包括血解素,运动性和基因表达.
主要成果:
- 非震动条件诱导了花,增加了生物膜的形成,以及更高的MAR值 (0.39),具有特定蛋白质分泌 (∼80和∼100kDa).
- 震动条件导致非花细胞,减少生物膜,较低的MAR值 (0.31) 和不同的蛋白质分泌 (∼50kDa).
- 非震动条件与减少毒性 (血解素,运动性) 相相关,但某些基因的表达更高 (saa, astA, ibeA, papC, papG),而震动条件显示出更高的毒性和其他基因的表达 (kpsMT K1, kpsMT "K5", stx1, ireA, cdt).
结论:
- 莫尔加尼表现出两种独特的表型形式,受流体剪切的影响,影响其抗性,机密性和毒性.
- 流体剪切操纵在水产养殖中为监测和控制多药耐药M. morganii提供了一个潜在的战略.
- 了解这些表型开关对于管理M. morganii感染及其在不同环境中的行为至关重要.
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