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解读宿主基因组,十二指甲粘膜基因和微生物群在调节的复杂特征中的协调作用
Fangren Lan1,2,3, Xiqiong Wang1,2,3, Qianqian Zhou1,2,3
1State Key Laboratory of Animal Biotech Breeding and Frontier Science Center of Molecular Design Breeding, China Agricultural University, Beijing, 100193, China.
Microbiome
|March 2, 2025
概括
基因表达显著影响肠道微生物,新的调节性指标揭示了它对的复杂特征的影响. 这突出了基因表达.
科学领域:
- 主体微生物群的相互作用
- 基因组学和转录基因组学
- 动物科学动物科学
背景情况:
- 主体遗传和肠道微生物组的相互作用是复杂的.
- 基因表达对微生物组成的具体影响需要进一步探索.
研究的目的:
- 研究母的宿主基因组,粘膜基因表达和肠道微生物群关系.
- 引入并应用可调节性 (r2) 的概念来量化基因表达的表型效应.
- 比较和人类之间的宿主微生物相互作用机制.
主要方法:
- 对705只母的宿主基因组,粘膜基因表达和肠道微生物群的综合分析.
- 微生物种群的遗传性 (h2) 的计算.
- 可调节性 (r2) 和微生物活性 (m2) 值的量化.
- 估计 cis (c2) 和 trans 遗传性 (t2).
主要成果:
- 特定的肠道微生物,如Christensenellaceae显示遗传性和与宿主基因组变异的相关性.
- 十二指管基因表达 (r2=0.17) 强烈影响了十二指管微生物,超过了宿主基因组的影响 (h2=0.02).
- 和人类表现出类似的基因微生物能力,但不同的微生物调节性.
- 跨作用基因基因位点显著影响基因表达,而不是 cis 作用基因位点.
- CHST14基因和Lactobacillus salivarius的相互作用通过胆盐酸酶调节影响了腹部脂肪沉积.
结论:
- 可调节性 (r2) 量化了基因表达对表型变异的贡献.
- 肠道基因表达在复杂特征的宿主-微生物相互作用中比宿主基因组学发挥更为关键的作用.
- 这些发现可以通过调节宿主-微生物相互作用,为改善的经济特征制定策略.
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