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Updated: May 24, 2025

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
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A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

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一个微流体平台,用于提取和分析细菌基因组DNA

Alex Joesaar1, Martin Holub1, Leander Lutze1

  • 1Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands. c.dekker@tudelft.nl.

Lab on a chip
|March 3, 2025
PubMed
概括

研究人员开发了一种微流体装置,可以在没有碎片化的情况下提取和分析细菌核子. 这种方法保留了脆弱的兆基量级DNA,用于研究基因组组织和潜在的人工染色体组装.

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相关概念视频

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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