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电子转移驱动的纳米酶通过协同信号放大策略来提高生物传感器的灵敏度

Zongyou Chen¹, Keyang Lai¹, Aoxue Wang²

¹State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China.

|March 3, 2025

概括

多分支金纳米颗粒 (MnAuNPs) 通过利用协同光热效应来增强横向流动免疫试验的灵敏度. 这种新的方法显著提高了对诸如大肠杆菌O157:H7.7等病原体的检测极限.

科学领域:

纳米技术纳米技术
生物感应是一种生物感应.
分析化学分析化学

背景情况:

传统的金纳米粒子 (AuNPs) 对于敏感的侧流免疫试验 (LFIA) 具有有限的亮度.
开发高度敏感和快速的检测方法对于点诊断至关重要.

研究的目的:

合成具有增强氧化酶类活性的多分支金纳米颗粒 (MnAuNPs).
开发一种使用MnAuNPs及其氧化产物的敏感LFIA,以改进光热检测.
为了检测大肠杆菌O157:H7高灵敏度和速度.

主要方法:

一合成Au核心-Mn外纳米结构的MnAuNPs.
利用MnAuNPs的类似氧化酶的活性将3,3',5,5'-四甲丁 (TMB) 氧化成光热剂 (ox-TMB).
实施级LFIA战略,将MnAuNPs和ox-TMB结合起来,用于协同光热检测.

主要成果:

MnAuNPs表现出显著的氧化酶类活性,有效氧化TMB.
MnAuNPs和ox-TMB之间的协同光热效应提高了检测灵敏度.
开发的级联LFIA实现了239 CFU mL-1的*E. coli* O157:H7的检测极限,比AuNPs-LFIA提高了37.21倍.
该试验提供了准确的结果,在25分钟内恢复率在82.63-111.67%之间,变化系数为4.28-14.19%.

结论:

多分支MnAuNP和ox-TMB为开发高度敏感的LFIA提供了一个有前途的平台.
这种方法扩大了生物传感策略,用于快速的临床检测.
级联LFIA显示了敏感病原体检测的巨大潜力.

关键词:

大肠杆菌埃舍里奇亚菌O157:H7 级联 LFIA LFIA 的时间.电子转移是电子的转移.具有类似氧化酶的活性.灵敏度灵敏度灵敏度灵敏度灵敏度有协同效应的效应.

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