通过复杂的同化,用植物性多糖体使酶固定.
Waritsara Khongkomolsakul1, Eunhye Yang1, Younas Dadmohammadi1
1Department of Food Science, College of Agriculture and Life Sciences, Cornell University, 243 Stocking Hall, Ithaca, NY, USA.
概括
植物性多糖类被用来固定同类动物中的植物酶,增强酶的稳定性和活性. 像pectin和alginate这样的特定多糖类显示出高的植物酶活性保留,为植物性饮食提供了功能成分.
科学领域:
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
- 食品科学 食品科学 食品科学
背景情况:
- 酶固定在工业应用中至关重要.
- 植物性多糖为酶稳定提供可持续的矩阵.
- 了解植物酶-多糖相互作用是优化固定化的关键.
研究的目的:
- 为了使植物酶不动使用植物性多糖在一个协体系统.
- 调查多糖类功能组 (硫酸盐与碳酸盐) 在酶活性恢复中的作用.
- 为了将分子相互作用与植物酶活性保留相关联.
主要方法:
- 植物酶与各种多糖类 (卡拉基南,点,酸盐) 的协.
- 分子对接以预测酶-多糖相互作用.
- 测量泽塔电位和FTIR光谱,以确认相互作用机制.
- 在同生物复合体中检测植物酶活性保留的测定.
主要成果:
- 在pH值4和12:1的植物酶与多糖合物比率下优化协.
- 静电相互作用和结合驱动协形成.
- 观察到高的植物酶活性保留 (>75%) 与低的甲氧化,酸和卡帕-卡拉基南.
- 由于活性部位的结合,IOTA和LAMBDA-卡拉基南的保留率较低.
结论:
- 基于植物的多糖类可以有效地固定植物酶.
- 多糖的结构和功能组显著影响酶活性保留.
- 这项研究为开发稳定,功能性的植物酶成分用于植物性应用提供了框架.
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