在海洋中,由 Pseudomonadota 进行的转-4-氧-L-proline 代谢
Yan Wang1, Zhen Wang1, Wen-Xiao Zhao1
1State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237 China.
Marine life science & technology
|March 3, 2025
概括
海洋细菌,包括Halomonas和Salinicola,可以分解转-4-氧-L-proline (T4LHyp). 这项研究揭示了T4LHyp代谢的独特途径,突出了Pseudomonadota.
科学领域:
- 微生物学 微生物学
- 海洋生物学 海洋生物学
- 生物化学 生物化学
背景情况:
- 自由的trans-4-hydroxy-L-proline (T4LHyp) 是从原蛋白和糖蛋白中衍生出来的.
- T4LHyp的海洋微生物催化剂在很大程度上是未知的.
- 陆地细菌通过特定的基因集群利用T4LHyp.
研究的目的:
- 隔离和描述能够利用T4LHyp的海洋微生物.
- 为了阐明T4LHyp在海洋细菌中的代谢途径.
- 确定参与海洋T4LHyp循环的关键细菌群.
主要方法:
- 从热水风口沉积物中分离利用T4LHyp的海洋细菌.
- 基因组,转录组和生物化学分析T4LHyp代谢.
- 对T4LHyp基因群分布的生物信息分析.
主要成果:
- 有五种海洋细菌菌株 (Halomonas sp. 5021,萨利尼科拉 (Salinicola) sp. 这种植物. 4072,Alteromonas spp.) 的研究结果. 他们被隔离了.
- 哈洛蒙纳斯 sp. 的一种. 5021使用T4LHyp作为碳和源;其他人使用它作为源.
- 确定了不同的T4LHyp代谢途径,涉及不同的酶集.
- 在Pseudomonadota中,T4LHyp基因集群很普遍,在各种海洋环境中发现.
结论:
- 海洋细菌拥有T4LHyp代谢的多种机制.
- 伪虫细菌是海洋T4LHyp回收和矿化中的关键参与者.
- 了解这些途径对于海洋生物地质化学循环至关重要.
更多相关视频
14:42Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Published on: September 23, 2021
5.6K
07:26Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
Published on: January 26, 2012
24.8K
相关概念视频
Carbon-dioxide Fixation
501
Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...
501
Anoxygenic Photosynthesis
942
Anoxygenic photosynthesis is a phototrophic process that captures light energy to drive carbon fixation without producing molecular oxygen. Unlike oxygenic photosynthesis, which utilizes water as an electron donor and releases oxygen, anoxygenic phototrophs use alternative electron donors such as hydrogen sulfide (H₂S), elemental sulfur (S⁰), or thiosulfate (S₂O₃²⁻). This process is carried out by diverse groups of bacteria, including purple bacteria, green...
942
Lipid Catabolism
714
Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
714
Amino Acid Catabolism
750
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
750
Metabolism of Chemolithotrophs
622
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
622
Anoxygenic Phototrophic Bacteria
620
Anoxygenic phototrophic bacteria are a diverse group of microorganisms that perform photosynthesis without producing oxygen. They primarily include purple sulfur bacteria, purple nonsulfur bacteria, green sulfur bacteria, and green nonsulfur bacteria. These bacteria are classified into the Gammaproteobacteria, Alphaproteobacteria, Betaproteobacteria, Chlorobi, and Chloroflexi lineages, each with distinct physiological and ecological adaptations.Purple sulfur bacteria belong to the...
620
