由于卵细胞中的凝聚性保护错误导致的混合雌性不育
Warif El Yakoubi1, Bo Pan1, Takashi Akera1
1Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health; Bethesda, Maryland 20894, USA.
bioRxiv : the preprint server for biology
|March 3, 2025
概括
雌性老鼠杂交物是无菌的,因为它们的卵在介质变异I过程中无法正确分离染色体. 这种生殖障碍可能会驱动物种化,并与人类不孕症有关.
科学领域:
- 生殖生物学 生殖生物学
- 遗传学 是一个遗传学.
- 进化生物学是进化的生物学.
背景情况:
- 杂交不相容性通过导致杂交致死性或不育性,有助于物种化.
- 缺陷的染色体分离在半体变化是已知的原因的形和不孕症.
研究的目的:
- 研究雌性小鼠中混合不孕症的遗传和分子基础.
- 确定凝聚蛋白调节在混合化I功能障碍中的作用.
- 探索对哺乳动物物种化和人类不孕症的影响.
主要方法:
- 介质化I的比较分析来自跨物种小鼠杂交体 (Mus musculus domesticus x Mus spicilegus) 的卵细胞中.
- 免疫光显微镜用于评估凝聚蛋白相关蛋白的局部化和功能 (SGO2,BUB1).
- 检查卵细胞和线性细胞中的染色体分离.
主要成果:
- 雌性杂交体会表现出不育性,原因是未能在化I过程中分离同类染色体,从而产生无体卵.
- 凝聚素保护剂SGO2的错误定位从中间体到染色体臂,导致过度保护.
- 混合卵细胞中BUB1激酶活性升高与SGO2错误准相关.
- 在Peromyscus属的杂交品种中观察到类似的凝聚力缺陷.
结论:
- 异常的凝聚力保护,由增加的BUB1活动驱动,导致杂交错误和鼠标杂交中的无菌性.
- 这些介质缺陷有助于繁殖隔离和物种化.
- 这些发现表明,人类不孕症的原因与哺乳动物物种化过程之间存在联系.
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