脊椎动物移动的远端的分子组织
Juyeon Hong1,2, Chanjae Lee1, Ophelia Papoulas1
1Department of Molecular Biosciences, University of Texas at Austin, Texas, USA.
bioRxiv : the preprint server for biology
|March 3, 2025
概括
两种新型蛋白质,Ccdc78和Ccdc33,组织和稳定脊椎动物多细胞 (MCC) 中移动的远端尖端. 它们的微管结合活动对于毛长度调节和整体毛功能至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 发展生物学 发展生物学
背景情况:
- 毛对多毛细胞 (MCC) 的打击对于动物的发育和平衡至关重要.
- 对于MCC乳毛片的远端尖端的分子组成的了解很少.
- 毛对于各种生理过程至关重要,它们的功能障碍导致毛病.
研究的目的:
- 为了描述脊椎动物MCC cilia的远端分子组织.
- 为了确定参与MCC乳毛远端端结构和功能的新型蛋白质.
- 研究这些蛋白质在毛长度调节和稳定中的作用.
主要方法:
- 利用青,老鼠和人类的MCC进行比较分析.
- 采用分子生物学技术来识别和描述蛋白质成分.
- 进行了体内和体外实验,以评估蛋白质功能,包括微管团结活性.
主要成果:
- 在MCC乳毛的远端发现了两个不同的域,具有独特的蛋白质组成.
- 确定了Ccdc78和Ccdc33作为局部在极端远端的关键蛋白质.
- 证明Ccdc78和Ccdc33对于其他尖端蛋白的组织 (Spef1,Cep104,Eb3) 和毛长度调节至关重要.
- 展示了Ccdc78和Ccdc33.3的微管结合活性.
结论:
- Ccdc78和Ccdc33形成了一个关键模块,用于组织和稳定脊椎动物MCC中移动的远端.
- 这些蛋白质对于正常的乳毛长度调节至关重要.
- Ccdc78和Ccdc33代表了运动性纤维病变的潜在疾病位置,突出了它们的临床意义.
相关概念视频
01:24Microtubules in Cell Motility
3.2K
Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
3.2K
01:05Mechanism of Ciliary Motion
3.6K
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
3.6K
01:22Microtubules in Signaling
1.7K
The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
1.7K
01:39Mechanism of Filopodia Formation
2.3K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.3K
01:31Mechanism of Lamellipodia Formation
2.5K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.5K
01:32Cytoskeletal Coordination in Cell Migration
4.7K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.7K