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断奶时的微生物继承是由宿主甘氨酸的微生物代谢指导的

Jean-Bernard Lubin¹, Paul J Planet^1,2,3, Michael A Silverman^1,2,3,4

¹Division of Infectious Disease, Department of Pediatrics, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA.

bioRxiv : the preprint server for biology

|March 3, 2025

概括

主体甘氨酸对奶期间肠道微生物的继承至关重要. 利用宿主甘氨酸有助于微生物殖民婴儿肠道,影响免疫发育.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
发展生物学发展生物学

背景情况:

断奶期间从牛奶过渡到固体食物对婴儿的肠道微生物群和免疫系统产生了深刻的影响.
了解驱动微生物群落在断奶时发生变化的因素,对于促进健康发育至关重要.

研究的目的:

研究微生物和宿主因素影响肠道微生物在断奶过渡期间的继承.
探索宿主糖甘利用在微生物殖民和免疫成熟中的作用.

主要方法:

同住的 gnotobiotic 鼠标与一个断奶前微生物群体 (PedsCom) 和一个成年人衍生的联盟 (OMM12) 共同定居.
微生物群落的基因组分析,以评估碳水化合物活性酶概况.
使用*Akkermansia muciniphila*进行体内验证,以确认粘素甘氨酸代谢的作用.

主要成果:

同居成功模仿了与断奶相关的微生物继承和免疫成熟.
来自成人联盟 (OMM12) 的微生物具有更高的宿主甘氨酸利用能力,是更成功的殖民者.
该PedsCom社区缺乏酶来降解宿主甘氨酸,如粘蛋白.
* Akkermansia muciniphila * 的殖民依赖于代谢粘素甘氨酸.

结论:

主体衍生的甘氨酸是微生物社区结构在断奶过渡期间的关键驱动因素.
微生物利用甘氨酸对于婴儿肠道的成功殖民至关重要.
准宿主甘氨酸为调节肠道微生物组和增强断奶婴儿免疫发育提供了一个潜在的策略.

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