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相关概念视频

CRISPR and crRNAs02:53

CRISPR and crRNAs

16.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.5K

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相关实验视频

Updated: May 24, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

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开发基于案例嵌入策略的安全高效的CGBE编辑器.

Tian Lin1,2,3, Xin Wang4, Yu Zhang4,5

  • 1Cancer Institute, Xuzhou Medical University, 209 Tongshan Road, Xuzhou, Jiangsu, 221004, China.

Synthetic and systems biotechnology
|March 3, 2025
PubMed
概括
此摘要是机器生成的。

我们开发了新的Cas嵌入式C-to-G基编辑器 (CE-CGBE),用于精确的DNA编辑. 高频-CGBE变种显示出高效率和纯度,具有最小的目标外影响,具有治疗潜力.

关键词:
在CGBE中,CGBE是最重要的.体嵌入式嵌入式远离目标,远离目标eA3AA 是一个

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Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
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Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
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相关实验视频

Last Updated: May 24, 2025

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Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
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科学领域:

  • 分子生物学分子生物学
  • 基因编辑技术的技术
  • 生物化学 生物化学

背景情况:

  • 氨酸到氨酸基编辑器 (CGBE) 精确地将氨酸转化为氨酸.
  • 传统的CGBE系统面临的挑战是由于cytidine deaminases的非特异性DNA结合,导致非目标效应.
  • 卡斯嵌入策略将功能性蛋白质集成到卡斯9中,以增强特异性并减少目标外突变.

研究的目的:

  • 为CGBE系统开创Cas嵌入策略.
  • 设计新的CGBE编辑器,称为CE-CGBE,以提高安全性和有效性.
  • 评估工程 CE-CGBE 编辑器,特别是 HF-CGBE 变体的性能.

主要方法:

  • 将案例嵌入策略应用于CGBE系统.
  • 新型CE-CGBE编辑器的工程,包括eA3A,RBMX和Udgx.
  • 评估HF-CGBE的编辑效率,纯度,分片形成和非目标效应 (DNA和RNA).

主要成果:

  • 工程 CE-CGBE 编辑器展示了高编辑效率和纯度.
  • 包括eA3A,RBMX和Udgx在内的HF-CGBE变种表现出卓越的性能.
  • 与DNA和RNA的负对照相比,HF-CGBE没有显示出非目标效应的显著差异.

结论:

  • 新的HF-CGBE编辑器显著扩展了基础编辑工具箱.
  • 开发的CE-CGBE系统为致病突变提供了一个有希望的治疗途径.
  • 案例嵌入策略有效地减轻了CGBE系统中的非目标效应.