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相关概念视频

Transgenic Plants02:50

Transgenic Plants

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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Plant Breeding and Biotechnology01:59

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Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
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Overview
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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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相关实验视频

Updated: May 24, 2025

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
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Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

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在植物转化中使用分割可选择标记物的新型基因堆叠方法.

Guoliang Yuan1, Md Torikul Islam2,3, Gerald A Tuskan2,3

  • 1Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, USA.

Bio-protocol
|March 3, 2025
PubMed
概括
此摘要是机器生成的。

我们开发了一种用于植物基因堆叠的新型分裂选择性标记系统,简化了使用单个抗生素选择多个基因引入的过程. 这种相互介导的方法加速了各种植物物种的遗传改进.

关键词:
阿拉比多普西斯 (Arabidopsis) 是一种植物.在英特因的基础上.工厂转换 工厂的转换 工厂的转换这是一棵树.蛋白质拼接是指蛋白质拼接.可以选择的标记.

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The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker
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The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker

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相关实验视频

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Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation
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Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
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科学领域:

  • 植物生物技术 植物生物技术
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 基因堆叠对于通过基因改造增强植物特征至关重要.
  • 传统的共同转化通常需要多个可选择的标记基因,使选择过程复杂化.
  • 用多种抗生素优化植物转变的选择可能具有挑战性.

研究的目的:

  • 开发一种高效和简化的基因堆叠在植物中的方法.
  • 为了克服在植物共同转化中使用多个可选择标记基因的局限性.
  • 为了促进多个基因在草木和木质植物中的引入.

主要方法:

  • 开发了一种使用两个二进制向量的整数介导分割可选择标记系统.
  • 每个载体都含有感兴趣的基因子集和部分可选择的标记基因片段.
  • 使用Agrobacterium介导的共同转化和选择与单个抗生素 (例如,卡纳米辛).

主要成果:

  • 成功地证明了单个可选择标记基因用于共同转换的使用.
  • 分裂标记系统使得植物中有效的基因堆叠成为可能.
  • 这种方法在草本植物和木质植物物种中被证明是有效的.

结论:

  • 这种因特因介导的分裂可选择标记系统简化了植物的共同转化和基因堆叠.
  • 这种方法加速了植物对多基因或多重特征的遗传改进.
  • 该系统提高了植物基因工程的可扩展性和可行性.