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Updated: May 24, 2025

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对tRNA衍生核酸的HPLC分析

Xingxing Chen1,2, Fu Xu3

  • 1Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Shanghai, China.

Bio-protocol
|March 3, 2025
PubMed
概括

这项研究提出了一种简化方法,用于分析酵母中的转移RNA (tRNA) 修饰. 该协议使用高性能液态染色学 (HPLC) 准确量化改性核化物,提供一个具有成本效益的替代方案.

关键词:
副转录ome 副转录ome 副转录ome 副转录ome在HPLC中使用HPLC.保存时间 保存时间它可以吸收紫外线,吸收紫外线.酵母酵母是一种酵母.tRNA的修改 tRNA的改变

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One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
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