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在Myxococcus xanthus纳米囊中进行表面工程,用于细胞向蛋白质输送

Sac Nicté Gómez-Barrera¹, Willy Ángel Delgado-Tapia¹, Aquetzali Estefanía Hernández-Gutiérrez¹

¹Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Av. Universidad 3000, CDMX, Mexico 04510, Mexico.

|March 3, 2025

概括

囊纳米隔间被设计成具有针对性的精确传递. 这些改造的蛋白质纳米显示出作为纳米医学先进的蛋白质输送载体的潜力.

科学领域:

生物技术是生物技术.
纳米医学是一种纳米医学.
蛋白质工程是指蛋白质的工程.

背景情况:

囊素 (ENCs) 是细菌/古生物蛋白质纳米,通过特定的具有固有的载荷能力.
异质蛋白质封装是可行的,突出显示ENCs是有前途的蛋白质载体.
针对性小组对于精确的细胞或组织输送ENCs是必要的.

研究的目的:

为了设计Myxococcus xanthus ENCs (MxENCs) 以提供有针对性的蛋白质.
为了使MxENC表面具有功能,使用生物直角联系统 (SpyTag/SpyCatcher).
通过使用PreS1来实现肝细胞特异向.

主要方法:

将SpyTag基因融合到特定的MxENC表面循环 (155-159) 和Enca的C端.
工程Enca的自组装成功能ENC.
由SpyCatcher融合的肝细胞向性 (PreS121-47) 与SpyTag修改的MxENCs的结合.

主要成果:

工程 EncA 蛋白自组装成稳定的 ENC.
通过SpyTag功能化的MxENCs成功与SpyCatcher-PreS1结合.
修改后的MxENCs在HepG2细胞中表现出稳定性,载荷,细胞吸收和载荷释放.

结论:

带有SpyTag/SpyCatcher系统的工程MxENCs能够提供有针对性的蛋白质.
修改后的纳米分区显示出作为多功能蛋白质输送载体的潜力.
这种方法为设计用于药物输送应用的定制纳米隔间提供了宝贵的见解.

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