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阐明在SARS-CoV-2变异中具有关心的尖端蛋白的阿普坦体的分子对接和结合动力学

Irwin A Quintela¹, Tyler Vasse¹, Dana Jian¹

¹Produce Safety and Microbiology Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Albany, CA, United States.

Frontiers in microbiology

|March 3, 2025

概括

研究人员开发了针对SARS-CoV-2变种的DNA吸管. 这些体表现出高结合亲和力,为针对COVID-19变种的诊断和治疗提供了潜在的潜力.

科学领域:

分子生物学分子生物学
生物技术是生物技术.
病毒学病毒学

背景情况:

引起关注的SARS-CoV-2变种 (VOCs) 构成了全球重大健康挑战.
DNA 体为针对病毒蛋白的抗体提供了一个有希望的替代品.
了解aptamer-S蛋白相互作用对于开发有效的对策至关重要.

研究的目的:

为了识别和表征具有高结合亲和力的DNA吸附体对VOCs的SARS-CoV-2尖端蛋白 (S-蛋白) 的结合亲和力.
评估这些体在诊断和治疗应用中的潜力.

主要方法:

通过指数式丰富 (SELEX) 的连接体的系统进化被用来选择DNA的体.
采用了in silico方法,包括结构预测和分子对接.
在实验室中使用直接酶相关的寡核酸测定 (ELONA) 进行表征,确定解离常数 (Kd).

主要成果:

成功识别了针对SARS-CoV-2 VOC S-蛋白的新型DNA阿巴特马序列.
在基分析中预测了阿普坦酶的结合和结构.
对于阿尔法,三角形和欧米克朗VOC亚体,ELONA试验显示了32nM193nM范围内的解离常数 (Kd).

结论:

选择的DNA体对SARS-CoV-2 VOC S-蛋白具有很高的特异性和亲和力.
这些体体代表了开发新型诊断工具和针对COVID-19变种的治疗策略的有希望的候选人.

关键词:

埃隆娜 (Elona) 是一个这就是SARS-CoV-2病毒.SELEX (通过指数级丰富的连接体的系统演化)它们是Aptamers.尖端蛋白质是指尖端蛋白质中的一个.

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