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化疗剂对表皮神经干细胞的影响

Nasim Rahmani-Kukia^1,2,3, Fatemeh Keshavarzi^4,3, Mohammad Saied Salehi⁵

¹Autophagy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Molecular biology research communications

|March 3, 2025

概括

化疗剂影响人类表皮神经干细胞 (hEPI-NCSCs). 一些像Dithiothreitol (DTT) 这样的药物激活了生存途径,而像5-Fluorouracil (5-FU) 和Cisplatin这样的药物则抑制了它们,影响了细胞迁移.

科学领域:

干细胞生物学干细胞生物学
分子生物学分子生物学
药理学药理学是指药理学的学科.

背景情况:

人类表皮神经干细胞 (hEPI-NCSCs) 是具有神经和非神经组织分化潜力的多能迁移细胞.
了解化疗剂对干细胞应激反应和迁移功能的影响,对于治疗开发至关重要.

研究的目的:

调查各种化疗剂在调节压力诱导的途径中的作用,特别是hEPI-NCSCs中的自和展开蛋白质反应 (UPR).
确定这些病原体对hEPI-NCSCs的迁徙潜力的影响.

主要方法:

治疗hEPI-NCSC患者的药物包括二二甲醇 (DTT),沙利诺米辛,埃布塞伦,5-甲 (5-FU) 和西斯普拉丁.
使用RT-qPCR分析了与自和UPR通路相关的基因表达.
细胞迁移能力通过伤愈合试验进行评估.

主要成果:

DTT和沙利诺米辛上调自和与UPR相关的基因 (LC3,P62,CHOP).
沙利诺米辛和西斯普拉丁上调了XBP-1,而DTT,5-FU和Ebselen则降低了XBP-1的调节.
沙利诺米辛,5-FU,埃布塞林和西斯普拉丁抑制了NCSC迁移;DTT和5-FU促进了BDNF表达,而沙利诺米辛,西斯普拉丁和埃布塞林减少了它.

结论:

自作为一种生存机制,对抗DTT诱导的NCSC迁移的抑制.
像5-FU和 Cisplatin这样的化疗剂通过抑制自,UPR通路和NCSC迁移潜力来产生细胞毒性作用.

关键词:

自自是一种自的过程.化疗剂化疗剂化疗剂神经干细胞神经干细胞不折叠的蛋白质反应

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