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循环上皮细胞作为病毒感染和组织起源标志物在患有严重COVID-19的患者中

Valeria Denninghoff^1,2, María Del Carmen Garrido-Navas¹, María Del Pilar Molina-Vallejo¹

¹Liquid Biopsy and Cancer Interception Unit, GENYO, Centre for Genomics and Oncological Research (Pfizer/University of Granada/Andalusian Regional Government), Granada, Spain.

The journal of liquid biopsy

|March 3, 2025

概括

液体活检 (LB) 在严重的COVID-19患者中检测到更高水平的循环上皮细胞 (CEC). 这种最少的侵入性方法对实时监测传染病进展有希望.

科学领域:

* * 临界护理医药临界护理医药
* 传染病研究研究.
* 分子诊断的方法.

背景情况:

* 液体活检 (LB) 通过体液生物标志物提供最少的侵入性监测.
*严重的COVID-19为实时患者状态评估带来了挑战.
*循环上皮细胞 (CEC) 是组织损伤的潜在指标.

研究的目的:

* 开发和评估一种新的LB方法来分析CEC.
*为了评估严重的COVID-19患者中CEC的存在和特征.
*为了将CEC的发现与SARS-CoV-2感染状况相关联.

主要方法:

*分析了重症监护室 (ICU) 中严重COVID-19患者的CEC.
*使用高暴露负面人群对COVID-19 (HENPC) 作为对照组.
*通过多光谱成像流细胞计对CEC进行表征.
*使用特定抗体检测SARS-CoV-2 Spike S1蛋白 (ProtS).

主要成果:

* 在大多数ICU患者中检测到CEC (p=0.0412).
* 在患者和对照组中观察到明显更高的CEC中位数 (p=0.0004).
*CEC集群是患者独有的;在患者的CEC中发现了高的ProtS表达.

结论:

* LB是一种可行的,最少侵入性的工具,用于检测传染病原体诱导的组织损伤.
* LB提供了对疾病状况和演变的实时生物见解.
*建议在更大的患者队伍中进一步验证.

关键词:

循环的上皮细胞循环.在重症监护病房的重症监护病房.液体活检液体活检蛋白质S 是一种蛋白质.这就是SARS-CoV2病毒.

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