在西班牙,人类和动物中SalmonellaEnteritidis的分子流行病学
Clara Samper-Cativiela1,2, Laura Torre-Fuentes1, Bernabé Diéguez-Roda3
1VISAVET Health Surveillance Centre, Complutense University of Madrid, Madrid, Spain.
Antimicrobial agents and chemotherapy
|March 3, 2025
概括
在西班牙,来自人类和动物的沙门氏菌菌株表现出明显的遗传特征,动物菌株更频繁地携带抗菌素耐药性基因. 这表明,各种来源有助于食物传播感染.
科学领域:
- 微生物学 微生物学
- 流行病学 流行病学
- 基因组学就是基因组学.
背景情况:
- 沙门氏菌 (Salmonella Enteritidis) 是人类胃肠炎的主要原因,通常与家禽有关.
- 在Salmonella Enteritidis中对诺基诺和安培素的新兴耐药性是越来越令人担忧的.
- 了解遗传多样性和耐药性模式对于公共卫生至关重要.
研究的目的:
- 分析来自西班牙各个来源的沙门氏菌 (Salmonella Enteritidis) 隔离物的多样性和抗菌素耐药性 (AMR).
- 研究AMR转移基因 (ARG) 和突变的分布.
- 重建西班牙沙门氏菌感染的流行病学.
主要方法:
- 对298种来自人类,牲畜,野生动物,食品和环境来源的Salmonella Enteritidis分离物的基因分析 (2002-2021年).
- 检测AMR传递基因 (ARG) 和基因突变在醇耐药性决定性区域 (QRDR).
- 等离子体复制体类型和表型抗性概况.
主要成果:
- 确定了两个主要基因组:基因组I (主要是人体隔离物) 是泛敏感的,而基因组II (主要是动物/食物/环境隔离物) 经常存在QRDR突变.
- ARG很少见,但主要在Clade II菌株中发现,通常与IncX1等离子体复制品相关.
- 动物分离物体的表型耐药性更高,但特定的耐药性基因 (ESBL,AmpC,卡巴酶) 仅在人类分离物体中发现.
结论:
- 在西班牙分离的人类和动物的沙门氏菌Enteritidis在很大程度上属于不同的基因组.
- 不同的来源,除了传统的家禽链接外,可能会导致食物传播感染.
- 特定的等离子体相关的ARG和毒性因子表明不同的进化动态驱动抗性选择.
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