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Updated: May 24, 2025

Flow Cytometric Characterization of Murine B Cell Development

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Published on: January 22, 2021

通过流式细胞计量对B细胞亚群的免疫类型鉴定

Michaela Fux¹, Joseena Iype²

¹University Institute of Clinical Chemistry, Inselspital, Bern University Hospital, Bern, Switzerland.

Methods in molecular biology (Clifton, N.J.)

|March 3, 2025

概括

本章详细介绍了一种流细胞测量协议,用于免疫表型B细胞子集. 准确分析B细胞种群有助于诊断免疫缺陷和监测疗法.

科学领域:

免疫学免疫学免疫学
临床诊断临床诊断临床诊断

背景情况:

流细胞计对于分析免疫细胞至关重要.
B细胞子集在免疫和疾病中起着关键作用.

研究的目的:

通过流式细胞计量 (flow cytometry) 来进行B细胞子集免疫型定型的标准化方案.
为研究和临床环境中准确的B细胞分析提供资源.

主要方法:

抗体面板选择的详细方法.
用于最佳B细胞分析的样本准备技术.
对B细胞亚种群的数据分析策略.

主要成果:

该协议可以准确地定量和定性地确定B细胞.
能够识别特定的B细胞子集用于诊断和研究目的.

结论:

这种流细胞计量协议对于理解B细胞多样性和功能至关重要.
它支持诊断常见变性免疫缺陷等疾病,并监测B细胞枯竭疗法.

关键词:

抗体面板中的抗体 B细胞的枯竭导致B细胞的枯竭.B 细胞子集 B 细胞子集流动细胞计量流动细胞计量免疫缺陷是一种免疫缺陷.免疫型定型免疫型定型监控监控监控监控

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相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...