一个全面的方法来使瘤透的B淋巴细胞从上皮癌中永生
Baishali Tamuli1,2, Subir Biswas3,4
1Cancer Immune Environment and Therapeutics Lab, Tumor Immunology and Immunotherapy, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, India.
Methods in molecular biology (Clifton, N.J.)
|March 3, 2025
概括
这项研究详细介绍了一种方法来隔离,不朽化和冷保存从上皮瘤中的B淋巴细胞. 这种技术支持抗体生产和癌症研究的基因分析.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 生物技术是生物技术.
背景情况:
- 表皮癌的特征是具有预后意义的T和B淋巴细胞.
- 了解瘤透淋巴细胞对于癌症研究和治疗至关重要.
研究的目的:
- 提供一个详细的协议,以隔离,扩张,不朽化和冷保存B淋巴细胞从上皮瘤.
- 为了实现下游应用,如抗体生产和遗传测序.
主要方法:
- 通过手术去除上皮瘤.
- 隔离可活性的B淋巴细胞.
- 淋巴细胞永生化和冷保存技术.
- 基因分析的测序方法.
主要成果:
- 这是一种强大的程序,可以从瘤组织中获得可行的,不朽的B淋巴细胞.
- 该方法促进了批量和抗原特异性抗体的生产.
- 能够对瘤透的B淋巴细胞进行基因测序.
结论:
- 描述的最先进的程序对于推进瘤免疫学研究至关重要.
- 这种方法支持与瘤透B细胞相关的发现的临床翻译.
- 促进开发针对上皮癌B细胞群的新型诊断和治疗方法.
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