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克里斯普尔元素及其与肠球菌中的宏化物和氨基糖化物抗性基因的关联

Rafael L do Rêgo¹, Felipe P G Neves¹, Filipe M Miranda¹

¹Instituto Biomédico, Universidade Federal Fluminense, Niterói, Brazil.

Microbial drug resistance (Larchmont, N.Y.)

|March 3, 2025

概括

在肠球菌中,集群定期间隔的短平行列重复 (CRISPR) 系统可能会防止抗菌素耐药性. 这项研究发现,CRISPR缺失与类和氨基糖化物耐药性基因相关,有助于理解耐药性演变.

科学领域:

微生物学微生物学
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

肠球菌是人类重要的病原体,经常携带抗菌素耐药性基因.
克里斯普尔-卡斯系统是 prokaryotic 免疫系统,可以防御外来遗传元素,包括编码抗生素耐药性的那些.

研究的目的:

调查CRISPR元素存在与*Enterococcus faecalis*和*Enterococcus faecium*中的抗菌素耐药性基因之间的相关性.
了解CRISPR在肠球菌中对类和高水平氨基糖化物耐药性 (HLR-A) 决定因素的获取和流行中的作用.

主要方法:

聚合酶链反应 (PCR) 用于检测特定的宏化物耐药基因 (erm(A), erm(B),HLR-A基因 (aac(6') -Ie-aph(2′′) -Ia, aph(2′′) -Ic, ant(6) -Ia) 和II-A型CRISPR元素.
使用双尾费舍尔精确测试来评估CRISPR与抗菌素耐药性基因存在的统计关联.

主要成果:

超过一半的分离物 (58.4%) 含有至少一个CRISPR元素,其中CRISPR3-Cas是最常见的.
在35.9%的分离物中检测到高水平的氨基糖化物耐药性基因,在38.9%的分离物中检测到高水平的氨基糖化物耐药性基因.
克里斯普尔基因的缺失与特定的HLR-A基因 (*aph(2′′) -Ic*, *ant(6) -Ia*) 和宏类耐药基因 *erm(B) *的存在有显著的关联 (p <0.05),特别是在缺乏CRISPR3-Cas.的*E. faecalis*中.

结论:

这项研究表明,克里斯普尔系统的缺失与肠球菌中关键的氨基糖化物和化物耐药性基因的存在之间存在显著的关联.
这些发现有助于阐明肠杆菌种群中抗微生物耐药性的进化动态,并突出显示了CRISPR在抗药性基因获取方面的潜在保护作用.

关键词:

克里斯普尔是什么意思?克里斯普尔是什么意思?肠球菌 (Enterococcus spp.) 是一种细菌.氨基甘油酸氨基化物.抗微生物耐药性抗微生物耐药性宏化物是什么?宏化物是什么?

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