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Updated: May 24, 2025

Tumor Hypoxia Assessment: In Vivo 3D Oxygen Imaging Through Electron Paramagnetic Resonance

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Published on: February 14, 2025

瘤缺氧评估:通过电子偏磁共振进行体内3D氧气成像

Gabriela Dziurman¹, Aleksandra Bienia¹, Aleksandra Murzyn¹

¹Faculty of Biochemistry, Biophysics and Biotechnology, Department of Biophysics and Cancer Biology, Jagiellonian University; Doctoral School of Exact and Natural Sciences Faculty of Biochemistry, Biophysics and Biotechnology, Department of Biophysics and Cancer Biology, Jagiellonian University.

Journal of visualized experiments : JoVE

|March 3, 2025

概括

电子磁共振成像 (EPRI) 在体内精确地绘制了瘤氧气水平. 这种技术与超声波相结合,通过量化缺氧,有助于评估癌症的攻击性和治疗反应.

科学领域:

生物医学成像技术生物医学成像技术
医学物理医学物理
在瘤学瘤学.

背景情况:

精确测量氧的部分压力 (pO2) 对于理解癌症病理至关重要,因为瘤缺氧与攻击性和治疗结果差相关.
量化瘤pO2有助于评估治疗有效性和监测疾病进展.

研究的目的:

评估脉冲电子磁共振成像 (EPRI) 与超声波 (US) 结合用于瘤体内氧气映射的实用性.
为了证明重复EPRI测量的可行性,用于纵向评估瘤氧化.

主要方法:

人类质母细胞瘤LN229瘤在小鼠中生长.
脉冲EPRI在给药后使用水溶性氧度自旋探针 (OX071) 进行.
超声波成像用于解剖参考,图像注册使用 MATLAB 软件进行.

主要成果:

EPRI提供了敏感和快速的三维 (3D) 组织氧化数据.
旋转探头的放松时间 (T1/T2) 准确地反映了pO2在选定的voxels.
联合EPRI-US方法允许计算缺氧分数并比较pO2分布图谱随时间的推移.

结论:

脉冲EPRI与超声波结合,为临床前瘤氧气测绘提供了一个强大的方法.
旋转探针的低毒性使重复测量成为可能,在治疗过程中促进动态监测瘤氧化.
这种综合成像方法在促进癌症研究和治疗评估方面具有重大潜力.

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