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在Sarcandra glabra中,的代谢由不同的BAHD氧胺基转移酶介导

Paul Bömeke¹, Maike Petersen¹

¹Institut für Pharmazeutische Biologie und Biotechnologie, Philipps-Universität Marburg, Robert-Koch-Str. 4, Marburg, 35037, Germany.

The Plant journal : for cell and molecular biology

|March 3, 2025

概括

研究人员确定了来自Sarcandra glabra的五个BAHD乙烯转移酶,涉及到代谢. 这些酶合成各种基酸,包括新型化合物如加密酸,进步我们对植物天然产品生物合成的理解.

科学领域:

植物生物化学植物生物化学
自然产品的生物合成.
酶学是一种酶学.

背景情况:

Sarcandra glabra表现出复杂的代谢与多种基酸的复杂代谢.
这种复杂性表明,多个氧氨基转移酶参与了它的生物合成.
BAHD乙转移酶是一种超级家族,以其在乙转移反应中的作用而闻名.

研究的目的:

为了识别和表征来自Sarcandra glabra的氧胺基转移酶.
阐明这些酶的特定基质和产物.
了解这些酶在植物的代谢中的作用.

主要方法:

从S. glabra的cDNA中放大了五个BAHD乙转移酶编码序列.
在大肠杆菌中蛋白质的异质表达.
生物化学测试以确定酶动力学和基质特异性.
分析不同S. glabra组织中的酶表达.

主要成果:

用已识别的基质和产物对SgHST,SgHQT1,SgHQT2和SgRAS酶进行表征.
SgHST形成p-coumaroyl/caffeoylshikimic酸,酸和氧氨酸胺基.
SgHQT1和SgHQT2合成特定的p-coumaroyl/caffeoyl酸,SgHQT2是第一个为加密酸前体的特征酶.
斯格拉斯合成迷酸及其前体,以及用d-氨基酸合成的胺.
第五种酶SgHCT-F没有发现基质.

结论:

来自S. glabra的五个BAHD乙烯转移酶已被确定并功能性地表征.
这些酶在各种基酸和相关化合物的生物合成中发挥着不同的作用.
参与加密酸生物合成的酶的发现为植物二次新陈代谢提供了新的见解.

关键词:

这就是BAHD的意思.类动物 (Chloranthales) 是一种类动物.萨坎德拉白 (Chloranthaceae) 植物中的一个.咖啡基基胺酸是咖啡基胺酸的一种.原酸是一种原酸.加密化酸是什么意思基锡纳米尔转移酶是什么的新陈代谢的代谢烯胺衍生物的衍生物.罗斯马林酸是一种酸.

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