主体免疫细胞膜的可变性控制了疟疾衍生的细胞外囊泡的吸收路径
Daniel Alfandari1, Irit Rosenhek-Goldian2, Ewa Kozela1
1Department of Biomolecular Sciences, Faculty of Biochemistry, Weizmann Institute of Science, Rehovot 7610001, Israel.
ACS nano
|March 3, 2025
概括
疟疾寄生虫释放出囊泡,以不同的方式感染免疫细胞. T细胞使用膜相互作用,而单细胞使用内细胞分裂,受细胞膜特性的影响.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 寄生虫学的寄生虫学
背景情况:
- 疟疾寄生虫Plasmodium falciparum利用细胞外囊泡 (EVs) 进行生长和宿主免疫细胞通信.
- 寄生虫衍生的EVs进入各种免疫细胞类型的机制尚不清楚.
研究的目的:
- 阐明Plasmodium falciparum EVs进入宿主T细胞和单细胞的独特入口路径.
- 为了研究控制免疫细胞内化EV的生物物理因素.
主要方法:
- 图像流细胞计和先进的光分析来追踪EV吸收.
- 原子力显微镜用于评估细胞膜的生物物理性质.
- 对细胞膜胆固醇含量进行操纵,以研究吸收动态.
主要成果:
- 寄生虫EV在T细胞 (血膜相互作用) 和单细胞 (内细胞分裂) 之间表现出不同的吸收机制.
- EV内部化路径受到内细胞结合和细胞膜生物物理性质的平衡的影响,而不仅仅是分子相互作用.
- 改变细胞膜胆固醇水平会使细胞膜相互作用和内细胞分裂之间的偏好转移为EV吸收.
结论:
- 提供了关于Plasmodium falciparum EVs如何进入特定宿主免疫细胞的机制见解.
- 这项研究揭示了疟疾寄生虫使用的复杂的细胞通信策略.
- 免疫细胞膜生物物理学在调节寄生虫EVs的内部化方面发挥着至关重要的作用.
相关概念视频
Intralumenal Vesicles and Multivesicular Bodies
3.4K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.4K
What are Membranes?
12.7K
A cell's plasma membrane demarcates the cell's borders and determines the nature of its interaction with the environment. Cells exclude certain substances, take in others, and excrete some others in controlled quantities. The plasma membrane must be flexible to allow certain cells, such as red and white blood cells, to change their shape while passing through narrow capillaries. These are the more obvious plasma membrane functions. In addition, the plasma membrane's surface carries...
12.7K
Receptor-mediated Endocytosis
103.8K
Overview
103.8K
Introduction to Membrane Traffic
6.8K
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
6.8K
Membrane Asymmetry Regulating Transporters
4.3K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
4.3K
Mechanisms of Membrane-bending
2.6K
The living membranes are flexible due to their fluid mosaic nature; however, their bending into different shapes is an active process regulated by specific lipids and proteins. The membrane bending can be transient as seen in vesicles or stable for a long time as in microvilli. Cells regulate the size, location, and duration of the membrane curvature.
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
2.6K


