[Lacticaseibacillus paracasei E6通过其代谢物乙酸和酸改善了斑马鱼中维诺雷尔宾诱导的免疫抑制]
X U Xinzhu1, Lina Guo1, Kangdi Zheng2
1Department of Clinical Nutrition, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, China.
概括
乳酸菌cillus paracasei E6通过产生短链脂肪酸 (SCFA) 来改善斑马鱼的化疗诱导的免疫抑制. 这些SCFA,包括酸和酸,有助于恢复免疫细胞的数量和功能.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 维诺雷尔宾化疗可以导致显著的免疫抑制,增加感染风险.
- 益生菌为抵消化疗引起的免疫缺陷提供了潜在的治疗策略.
- 了解益生菌作用机制对于开发有效的免疫疗法至关重要.
研究的目的:
- 在斑马鱼模型中研究Lacticaseibacillus paracasei E6改善维诺雷尔宾诱导的免疫抑制的机制.
- 为了确定L. paracasei E6的特定代谢物,负责其免疫调节作用.
主要方法:
- 斑马鱼被用光素异硫酸 (FITC) 标记的L. paracasei E6殖民,以追踪肠道中的存在.
- 通过分析维诺雷尔宾治疗斑马鱼的免疫细胞群 (巨细胞,中性粒细胞,T淋巴细胞) 和细胞因子表达 (IL-12,IFN-γ) 来评估免疫调节活性.
- 使用LC-MS/MS.分析L. paracasei E6发酵超沉和斑马鱼代谢物中的短链脂肪酸 (SCFA).
- 在斑马鱼模型中评估了特定的SCFA (乙酸盐,酸盐,丁酸盐) 对免疫抑制的影响.
主要成果:
- 标有FITC标签的L. paracasei E6成功殖民了斑马鱼的肠道.
- L. paracasei E6治疗显著恢复了巨细胞和中性粒细胞的数量,增强了T淋巴细胞的光,并在免疫抑制的斑马鱼中增强了IL-12和IFN-γ的表达.
- 发酵L. paracasei E6产生显著更高水平的乙酸,酸和黄油酸,这也被检测到治疗斑马鱼.
- 酸和 propionate 治疗增加了免疫细胞的数量,并抑制了胸腺T细胞的减少.
结论:
- 在斑马鱼中,Lacticaseibacillus paracasei E6有效地改善了维诺雷尔宾诱导的免疫抑制.
- 免疫调节作用是由其短链脂肪酸代谢产物,特别是酸和酸,调节的.
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