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生物和制药应用的光学C追踪使用双色腔环缩光谱
Jun Jiang1, David Baliu-Rodriguez2, A Daniel McCartt1
1Center for Accelerator Mass Spectrometry, Lawrence Livermore National Laboratory, Livermore, California 94550, United States.
Analytical chemistry
|March 3, 2025
概括
基于激光的碳-14 (14C) 量化使用双色腔环降光谱 (2C-CRDS) 为AMS提供了一种灵敏,负担得起的替代方案. 这种中红外激光技术可以在生物和制药研究中实现C追踪的子fCi量化.
科学领域:
- 分析化学 分析化学
- 频谱学是一种光谱学.
- 同位素追踪 (Isotope Tracing) 是一种同位素追踪方法.
背景情况:
- 加速器质谱 (AMS) 是14C定量测量的标准,但成本昂贵,吞吐量低.
- 基于激光的方法提供了一个潜在的更容易获得和更有效的替代方案.
- 双色腔环降光谱 (2C-CRDS) 已经显示出对14CO2的敏感光学测量有希望.
研究的目的:
- 为了证明中红外2C-CRDS检测器在生物和制药应用中用于低级C同位素追踪的可行性.
- 评估2C-CRDS系统的灵敏度,准确度和吞吐量,以进行14C量化.
- 评估2C-CRDS作为AMS的成本效益高,高吞吐量替代品的潜力.
主要方法:
- 使用中IR 2C-CRDS系统测量来自燃烧样本的CO2吸收信号.
- 将2C-CRDS C传感器应用于生物化学分析和制药研究.
- 研究了一种流通CO2采样方案以提高测量吞吐量.
主要成果:
- 实现了与AMS相匹敌的检测灵敏度和定量精度,在每万亿14个C/C分子分数中的几部分.
- 证明了样本C活动的fCi以下水平量化,样本最小样本大小为3毫克的碳.
- 目前的吞吐量为~25分钟/样本,受到样本处理的限制,有可能提高到每样本几分钟.
结论:
- 2C-CRDS C传感器是一种可行的,具有竞争力的低水平同位素追踪技术.
- 该系统提供高灵敏度和精度,使其适合生物,制药和临床研究.
- 由于提高效率,降低成本和紧的尺寸,有可能彻底改变C跟踪.
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