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从ONT草稿基因组数据获取新型细菌生物合成潜力

Marco A Campos-Magaña^1,2, Vitor A P Martins Dos Santos^1,3, Luis Garcia-Morales³

¹Dept. Bioprocess Engineering, Wageningen University and Research, Wageningen, the Netherlands.

Microbial biotechnology

|March 4, 2025

概括

研究人员使用牛津纳米孔技术 (ONT) 测序和抗SMASH开发了一种具有成本效益的管道,用于识别和克隆来自土壤细菌的新型天然产品生物合成基因集群 (BGC).

科学领域:

自然产品的发现自然产品的发现
基因组学就是基因组学.
合成生物学合成生物学

背景情况:

自然产品提供了多样化的生物活动,但很难获得.
土壤细菌是新型自然产品的丰富来源.
目前的方法限制了这些有价值的化合物的发现.

研究的目的:

开发一个高效和成本效益的管道,以获取来自土壤细菌的生物合成基因集群 (BGC).
为了利用长读测序来改进BGC识别和克隆.
能够发现具有潜在治疗应用的新型天然产品.

主要方法:

利用牛津纳米孔技术 (ONT) 进行长读测序,用于基因组组装.
采用反SMASH软件用于识别BGCs.
应用转化相关重组 (TAR) 克隆用于BGC隔离.
在myxobacteria Aetherobacter fasciculatus SBSr002.2.上对管道进行了测试.

主要成果:

证明了ONT测序和antiSMASH在识别新型BGCs方面的有效性,即使面临高GC含量等挑战.
从A. fasciculatus SBSr002成功地克隆了一个以前未知的BGC,变成了基因组工程准备的载体.
验证了管道的快速和低成本BGC发现和隔离能力.

结论:

开发的管道提供了一种强大且具有成本效益的策略,用于从土壤细菌获取BGC.
这种方法显著提高了新型自然产品的发现潜力.
促进了BGCs的隔离,以便进一步研究和潜在的生物技术应用.

关键词:

生物信息学是一种生物信息学.微生物基因组学微生物基因组学自然产品是自然产品的产品.二次代谢产物二次代谢产物合成生物学合成生物学

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