在致病性细菌上,Trichoderma asperellum培养过物的抑制作用,Burkholderia pseudomallei
Naritsara Roopkhan1, Thotsapol Chaianunporn1, Sorujsiri Chareonsudjai2
1Department of Environmental Science, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand.
特里科德玛·阿斯佩雷勒培养过物有效地杀死伯克霍德里亚 pseudomallei,并抑制生物膜的形成. 这种生物控制剂显示出降低B. pseudomallei在环境中的水平,并防止有机粉症的潜力.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 生物技术是生物技术.
背景情况:
- Burkholderia pseudomallei 致使有机,这是一种通过环境暴露获得的危及生命的感染.
- 减少环境中的B. pseudomallei对于预防有机粉症至关重要.
- 特里科德玛·阿斯佩雷勒 (Trichoderma asperellum) 是一种被称为生产抗菌物质的真菌.
研究的目的:
- 为了研究T. asperellum培养过剂对B. pseudomallei的抗菌活性.
- 评估T. asperellum培养过物的抗生物膜功效.
- 探索T. asperellum作为对B. pseudomallei.生物控制剂的潜力.
主要方法:
- 阿加尔井扩散试验以评估抗菌活性.
- 确定最小抑制度 (MIC) 和最小杀菌度 (MBC).
- 时间杀伤测试,晶体紫色染色和扫描电子显微镜 (SEM) 来评估抗生物膜效应和机制.
主要成果:
- 来自7天培养的T. asperellum过液 (TD7) 显示了B. pseudomallei (30.33毫米区域) 的显著抑制.
- TD7具有抗菌活性,MIC为7.81 mg/mL,MBC在7.81-11.72 mg/mL之间.
- TD7有效地减少了B. pseudomallei生物膜的形成,并导致细胞膜的破坏.
结论:
- T. asperellum培养过物对B. pseudomallei具有强大的杀菌特性.
- 阿斯佩雷勒姆菌有效地抑制了B. pseudomallei生物膜的形成.
- T. asperellum 是一个有前途的候选人,用于环境控制B. pseudomallei,以减少粉症的发生率.
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