一代人的游戏:朝着连续文化的代代遗传稳定
Andrew Yiakoumetti1, Charlotte Green1, Mark Reynolds2
1Sustainable Process Technologies Research Group, Faculty of Engineering, University of Nottingham, Nottingham NG7 2RD, UK.
iScience
|March 4, 2025
概括
通过使用工程微生物,在1000多个小时内实现了甲酸盐 (CMA) 的持续生物生产. 这种方法提高了从可再生原料中可持续化学制造的体积生产率.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 可持续化学 可持续化学
背景情况:
- 使用工程微生物的Fed-batch生物工艺对于来自可再生原料的生物制品是常见的.
- 料批次培养的低体积生产率限制了低价值化学品的商业可行性.
- 连续培养提供了更高的生产力,但面临着工程菌株遗传不稳定的挑战.
研究的目的:
- 为了证明稳定,高产量的持续生物制造酸盐 (CMA).
- 为了克服工业应用工程微生物菌株的遗传不稳定性问题.
- 评估可持续化学品的持续生物处理的技术经济可行性.
主要方法:
- 为合成甲酸盐 (CMA) 开发工程微生物菌株.
- 连续培养 (化疗机) 培养超过1000小时.
- 等离子体稳定性策略包括*infA*补充和营养素限制 (酸盐与葡萄糖).
- 使用构成性促进剂以避免昂贵的诱导剂.
- 血传播基因表达与染色体基因表达的比较.
主要成果:
- 在1000多个小时内实现了CMA的连续生物生产.
- 达到0.32gCMAgDCW-1 h-1的体积生产率.
- 通过*infA*-补充确保了等离子体分离稳定性.
- 在酸盐限制下获得结构性等离子体稳定性;葡萄糖限制引起的不稳定性.
- 血传播的CMA合成酶表达产生了比染色体整合剂更高的生产力.
结论:
- 持续培养是可持续化学品高生产率生物制造的可行策略.
- 化学定位器中的酸盐限制有效地稳定了工程塑体,克服了连续处理的关键障碍.
- 这项工作提高了使用工程微生物用于可持续化学生产的技术经济可行性.
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