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影响和调节外种CD1实验室小鼠 (Mus musculus) 的雌激循环

Clare Freer, Nicolas M Orsi

Journal of the American Association for Laboratory Animal Science : JAALAS

|March 4, 2025

概括

惠顿效应同步了小鼠的雌激周期,但成功率很低. 接触活着的雄性或哺乳雌性显著改善了小鼠的受感性,提供了更好的同步策略.

科学领域:

生殖生物学生殖生物学
动物行为动物行为
实验室动物科学实验室动物科学

背景情况:

惠顿效应通过使用雄性激素同步了小鼠的雌性循环.
目前的方法只能达到50%的怀孕/假怀孕率.
优化同步对于高效的实验室研究至关重要.

研究的目的:

为了研究影响小鼠雌激周期同步的因素.
评估初始循环阶段对受体性的影响.
为了确定最佳的激素刺激来增强雌性小鼠的受感性.

主要方法:

实验1:按雌性周期阶段 (前雌性,雌性,雌性,雌性,雌性) 分组小鼠 (n=27).
实验2:将小鼠 (n=45) 暴露在各种刺激中 (假的,雄性床上用品,活的雄性,孕妇/哺乳期的雌性).
每天进行阴道细胞检查,持续21天,以监测雌性周期.

主要成果:

初始周期阶段没有影响第三天的受体性;观察到同步.
雌性活动的峰值发生在第6天和第7天.
与对照组相比,暴露于活着的雄性和哺乳雌性显著增加了受体.

结论:

目前的惠顿效应协议可以改进,以提高效率.
替代住房和费洛蒙暴露 (活雄,哺乳雌) 增强了老鼠的雌激素同步.
这些发现支持改善动物福利和研究可重复性.

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