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NAT10调节了囊胞基因组的激活,并调节了从毛囊到胚胎细胞的过渡过程

Zhi Cui¹, Cong Fu¹, Di Ai¹

¹Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education, First Hospital of Jilin University, Jilin University, Changchun, China.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

|March 4, 2025

概括

N-乙转移酶10 (NAT10) 对于小鼠的植入前发育至关重要. 它驱动着囊胚基因组激活 (ZGA) 和从毛囊转向母细胞细胞的转变,这对早期胚胎生长至关重要.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
发展生物学发展生物学
分子生物学分子生物学

背景情况:

N-乙转移酶10 (NAT10) 催化RNA N4-乙基提丁 (ac4C) 修饰,涉及各种疾病.
在植入前胚胎发育中RNA ac4C的作用需要进一步研究.

研究的目的:

调查RNAac4C修饰的功能,特别是NAT10,在小鼠植入前胚胎发育中的功能.
阐明NAT10在关键发育过渡过程中的作用,如囊胚基因组激活 (ZGA) 和从毛囊到母细胞细胞的过渡.

主要方法:

利用Nat10siRNA微注射来评估对早期胚胎的淘汰效应.
产生的生长卵细胞阶段特定的Nat10淘汰小鼠 (Zp3-Nat10lox/lox) 进行深入分析.

主要成果:

通过siRNA的Nat10敲击导致了菌阶段的停止,并降低了NANOG/CDX2表达,降低了Nanog mRNA的稳定性.
Zp3-Nat10lox/lox雌性小鼠表现出完全的无菌性,胚胎在2细胞阶段停止发育.
从Zp3-Nat10lox/lox小鼠的2细胞胚胎中观察到母亲mRNA降解和ZGA的缺陷.

结论:

在早期小鼠胚胎中,NAT10对于成功激活胚胎基因组 (ZGA) 是不可或缺的.
NAT10在胚芽细胞向胚芽细胞过渡过程中发挥着关键作用,对胚芽细胞形成至关重要.
这些发现凸显了NAT10在小鼠植入前发育过程中的关键调节功能.

关键词:

在 NAT10 中,NAT10 包含了NAT10.RNA N4 - 乙基丁的使用.胚胎发育过程中的胚胎.鼠标鼠标鼠标鼠标紫毒基因组激活活动

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