孕期晚期母亲过度营养会改变新生儿小子丸中的基因表达和组织形态学
Alex M Trauner1, Sarah R McCoski1, M Carey Satterfield2
1Department of Animal & Range Sciences, Montana State University, Bozeman, MT 59717, USA.
Journal of animal science
|March 4, 2025
概括
在马的晚期怀孕期间,母亲的过度营养会显著影响新生儿小的丸发育,导致丸更重,管更少. 这表明男性后代的潜在长期生殖健康影响.
科学领域:
- 马的生殖生理学 马的生殖生理学
- 发育生物学是发展生物学.
- 营养科学 营养科学
背景情况:
- 怀孕期间母亲的营养对胎儿的发育至关重要.
- 孕产妇过度营养对马匹男性后代生殖健康的影响尚不清楚.
- 了解这些影响对于马匹的繁殖和繁殖成功至关重要.
研究的目的:
- 研究母亲过度营养对新生儿马子丸发育的影响.
- 为了确定丸组织学和基因表达的特定变化,由母亲的饮食过量造成的.
主要方法:
- 从怀孕235天开始,16只四分之一马匹被分配到控制或过度养 (40%以上控制) 的饮食中.
- 产后从雄中收集了测试,用于组织形态测量分析和RNA测序.
- 统计分析包括SAS和R包用于差异基因表达和途径分析.
主要成果:
- 过度养小的试验比对照组重61% (P < 0.01).
- 过度养的小猪每场精卵管的数量显著减少 (P = 0.01),精卵管占据的丸面积的百分比较低 (P = 0.05).
- RNA测序确定了56个与类固醇生成,细胞调节和能量代谢相关的不同表达的基因.
结论:
- 孕期晚期母亲的过度营养会改变马中的胎儿丸发育.
- 这些变化包括丸体重增加和管管密度减少.
- 观察到的变化表明可能对雄性马匹生殖功能产生长期影响.
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