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Updated: May 24, 2025

Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells
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Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

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通过固定他的标签融合蛋白技术对抗伪过敏剂的MrgX2-向接体屏幕

Zhaomin Xia1, Qiumei Zhu2, Yi Shan1

  • 1School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.

Journal of medicinal chemistry
|March 4, 2025
PubMed
概括

开发了一种新的细胞膜染色学方法,用于选与Mas相关的G蛋白结合受体X2 (MrgX2) 抗体,识别具有显著反伪过敏活性的Z-3578化合物.

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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