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Updated: May 24, 2025

Extraction of Extracellular Vesicles from Whole Tissue

Extraction of Extracellular Vesicles from Whole Tissue

Published on: February 7, 2019

TcEVdb:来自单细胞转录组的T细胞衍生小细胞外囊泡的数据库

Tao Luo^1,2, Wen-Kang Shen^2,3, Chu-Yu Zhang^2,3

¹College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.

Database : the journal of biological databases and curation

|March 4, 2025

概括

来自T细胞的细胞外囊泡 (TcEVs) 对于免疫反应至关重要. 新的TcEVdb数据库揭示了TcEV异质性,并确定了淋巴瘤等疾病的潜在生物标志物.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

来自T细胞的细胞外囊泡 (TcEVs) 在免疫调节和瘤微环境调节中至关重要.
了解TcEV异质性受到当前EV分析技术和数据稀缺的限制.

研究的目的:

开发TcEVdb,这是一个全面的数据库,用于探索TcEV表达和集群,使用T细胞单细胞RNA测序数据.
为分析TcEV转录组及其与细胞起源的关系提供工具.

主要方法:

在T细胞单细胞RNA测序数据上使用SEVtras方法构建TcEVdb.
整合了来自51种T细胞类型的277,265个EV滴,跨221个样本,9个组织来源和23个疾病条件.
开发用于数据探索,可视化和基因查询的浏览和搜索模块.

主要成果:

TcEVdb包含了来自不同T细胞类型和条件的TcEV的广泛数据.
分析显示,扩散大B细胞淋巴瘤中CD4+ T细胞耗尽时,EV分泌量增加.
确定了与细胞运动性和线粒体功能相关的基因的独特TcEV集群.

结论:

TcEVdb是研究TcEV转录组的宝贵资源.
该数据库有助于EV基础的诊断和治疗各种疾病的进展.

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