Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

7.4K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
7.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Pinhole engineering based enhanced resolution (PEER) for fluorescence lifetime imaging microscopy.

Communications biology·2026
Same author

Association Between Biologic Therapy and Reduced Acute Rhinosinusitis Episodes: A Retrospective Database Study.

Laryngoscope investigative otolaryngology·2026
Same author

PSF-Driven Spatio-Temporal Blending in Fluorescence Lifetime Imaging Microscopy and Its Mitigation via Mean-Shift Super-Resolution-Based Masking.

bioRxiv : the preprint server for biology·2026
Same author

Neoadjuvant Immunotherapy in the Management of Cutaneous Squamous Cell Carcinoma.

Skin therapy letter·2025
Same author

Single-aperture SLAM microscopy with amplitude-tailored vector beams.

bioRxiv : the preprint server for biology·2025
Same author

Evaluation of Disparities in Management of Chronic Rhinosinusitis by Race and Ethnicity: An All of Us Research Program Study.

American journal of rhinology & allergy·2025
Same journal

An unusual extra-axial supratentorial presentation of atypical teratoid/rhabdoid tumor in a child.

Journal of neuropathology and experimental neurology·2026
Same journal

Primary intracranial sarcoma, DICER1-mutant: 5 cases highlighting variable clinical and molecular features.

Journal of neuropathology and experimental neurology·2026
Same journal

Novel YWHAE-NTRK3 fusion in a pediatric glioma.

Journal of neuropathology and experimental neurology·2026
Same journal

Comprehensive clinicomolecular characterization of a methylation-defined supratentorial high-grade astrocytoma with piloid features in a young adult with confirmed germline NF1 mutation.

Journal of neuropathology and experimental neurology·2026
Same journal

Spatial mapping, isolation, and culture of primary human microglia and astrocytes: A systematic review and framework for glial cell-based models.

Journal of neuropathology and experimental neurology·2026
Same journal

Multiparameter flow cytometry of CSF identifies elevated CD8+ effector memory and TEMRA T-cells in immune-mediated neurologic disorders.

Journal of neuropathology and experimental neurology·2026
查看所有相关文章

相关实验视频

Updated: May 24, 2025

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research
15:18

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Published on: January 12, 2013

16.3K

最大对比投影:用于生物医学图像堆分析的强大工具.

Nat Adamian1, Christopher Guirguis1, Ansel Link1

  • 1Surgical Photonics & Engineering Laboratory, Massachusetts Eye and Ear, Harvard Medical School, Boston, MA, United States.

Journal of neuropathology and experimental neurology
|March 4, 2025
PubMed
概括
此摘要是机器生成的。

与最大强度投影相比,最大对比投影在神经组织的3D显微镜中增强了图像对比度. 这种技术可以改善对小鼠外围神经的无标签成像的可视化.

关键词:
光是一种光.组织学 组织学没有标签的显微镜.周围神经和周围神经.阶段对比相位对比的相位对比.

更多相关视频

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM
09:25

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM

Published on: May 8, 2020

10.5K
A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells
12:49

A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells

Published on: September 28, 2019

12.7K

相关实验视频

Last Updated: May 24, 2025

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research
15:18

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Published on: January 12, 2013

16.3K
Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM
09:25

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers MADM

Published on: May 8, 2020

10.5K
A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells
12:49

A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells

Published on: September 28, 2019

12.7K

科学领域:

  • 显微镜的使用方法
  • 生物医学成像技术 生物医学成像技术
  • 神经科学是一个神经科学.

背景情况:

  • 最大强度投影 (MIP) 是一种常见的技术,用于将3D图像数据减少到2D.
  • MIP受到低信号噪声比和高背景的限制,可能会掩盖细节.
  • 需要改进的对比方法来详细分析生物结构.

研究的目的:

  • 为了评估最大对比度投影 (MCP) 与MIP相比提高图像对比度的有效性.
  • 为了证明MCP在神经组织的3D相对比和定量相位成像中的实用性.
  • 通过提供开源算法来促进MCP的采用.

主要方法:

  • 使用3D光,相对照和定量相显微镜,对新鲜冷的小鼠坐骨神经部位进行了成像.
  • 使用最大强度投影 (MIP) 和最大对比度投影 (MCP) 处理图像.
  • 开发了一个用户友好的,开源的基于Python的MCP算法.

主要成果:

  • 与MIP相比,最大对比度投影 (MCP) 的图像对比度明显更高.
  • 在定量阶段成像中增强的对比度使得神经形态的无标签多色成像成为可能.
  • MCP有效地改善了神经组织学样本的可视化.

结论:

  • 最大对比度投影为神经组织3D显微镜的最大强度投影提供了优越的图像对比度增强.
  • 在神经科学中,MCP是无标签成像和详细形态分析的宝贵技术.
  • 提供的开源算法促进了MCP在生物医学研究中的更广泛应用.