一个便携式的微流体装置,集成酶和色度测定,用于现场检测冷解的肉类
Tingxuan Gao1, Nan Wu1, Zerun Zhao1
1State Key Laboratory of Meat Quality Control and Cultured Meat Development, MOST,Nanjing Agricultural University, Nanjing, 210095, China; Key Laboratory of Meat Processing, MARA, Nanjing Agricultural University, Nanjing, 210095, China; Jiangsu Collaborative Innovation Center of Meat Production, Processing and Quality Control, Nanjing Agricultural University, Nanjing, 210095, China; College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
Biosensors & bioelectronics
|March 4, 2025
概括
在便携式设备上使用的新的HADH酶测定可以快速检测解的肉类造. 这种低成本的方法使用肉精液进行快速的现场测试,以确保肉的真实性.
科学领域:
- 食品科学 食品科学 食品科学
- 分析化学 分析化学
- 生物技术是生物技术.
背景情况:
- 用解的肉来改肉类是一个重要的经济和质量问题.
- 需要快速,具有成本效益的方法来区分新鲜和解的肉.
研究的目的:
- 开发一种快速的现场方法来区分解肉和新鲜肉.
- 将基于β-hydroxyacyl-CoA脱酶 (HADH) 的色度测定集成到便携式微流体布式分析装置 (μCAD) 中.
主要方法:
- 在家制造的基于HADH的μCAD的制造.
- 使用一种色度测定方法,使用 thiazolyl蓝和 phenazine methosulfate.
- 用肉精液进行测试以进行快速分析.
主要成果:
- 基于HADH的测试表明,它能够很好地适应各种解的肉类,并且干扰最小.
- CAD成功地区分了新鲜和解的肉样品.
- 整个检测过程在5分钟内完成.
结论:
- 开发的基于HADH的μCAD提供了一个方便和快速的 point-of-care测试解决方案.
- 这种方法具有很高的潜力,可以在现场检测解的肉,并防止伪造.
- 该测量有效地确保了肉的真实性和质量控制.
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