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功能性识别二烯合成酶,探索二烯结构多样性在Isodon的风景

Xinqi Song¹, Yanying Chen², Guanghong Cui²

¹Academician Workstation, Jiangxi University of Chinese Medicine, Nanchang, 330004, China; State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China.

Plant physiology and biochemistry : PPB

|March 4, 2025

概括

研究人员在Isodon属中探索了二烯合成酶的多样性,发现了新的生物合成途径和骨. 这项工作扩大了我们对天然产品生物合成的理解,并为新药发现提供了潜力.

科学领域:

植物生物化学植物生物化学
自然产品的生物合成.
分子生物学分子生物学

背景情况:

伊索登属是丰富的ent-kaurane类型二甲烯的来源,其中ent-kaurene和miltiradiene被充分研究.
之前对伊索登二烯合成酶的研究仅限于单一物种,阻碍了对其多样性的全面理解.

研究的目的:

系统地挖掘和功能性验证来自三种Isodon物种的二二烯合成酶.
通过转录和代谢学数据阐明这些酶在迪特尔生物合成中的作用.
为了识别Isodon属内的新型二烯骨架及其生物合成途径.

主要方法:

对三种Isodon物种进行了转录组和代谢组分析.
与迪特尔生物合成相关的基因表达特征被确定并与代谢组数据集成.
在大肠杆菌中重建了代谢途径,以对二二烯合成酶的功能特征进行鉴定.

主要成果:

11个二二烯合成酶的功能特征.
为几种二烯骨架,包括已知的如-烯和米尔蒂拉迪烯的生物合成途径被阐明.
在Isodon中首次报告了四种新的二烯骨架 (ent-13-epi-sandaracopimaradiene,ent-neoabietadiene,abieta-8(14) -en-13-ol合成酶和sandaracopimaradiene).

结论:

这项研究揭示了对Isodon属中二烯多样性的分子基础的新见解.
这些发现为开发新的生物活性分子和潜在的药物线索提供了宝贵的资源.
迪特尔合成酶的功能特征为未来的代谢工程工作提供了基础.

关键词:

生物合成网络的生物合成网络.迪特尔合成酶的使用方法功能多样化的功能多样化.这就是Isodonodon.

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