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氧化应激是由新出现的氧二尼特消毒副产品和它们与酶的结合相互作用引起的
Shaoyang Hu1, Fangyuan Dong1, Xiangxiang Li1
1School of Environmental Science and Engineering, Shandong University, China-America CRC for Environment & Health, Qingdao, 266237, China.
Journal of environmental management
|March 4, 2025
概括
新出现的饮用水污染物,氧基酸 (HHBNs),通过破坏抗氧化剂系统和破坏DNA,导致细胞损伤. 它们的细胞毒性与抑制酶 (CAT) 活性有关.
科学领域:
- 环境化学环境化学
- 毒理学 毒理学 毒理学
- 生物化学 生物化学
背景情况:
- Halohydroxybenzonitriles (HHBNs) 是饮用水中发现的含的芳香消毒副产品.
- 定量结构-活性关系模型表明,HHBN细胞毒性可能涉及氧化应激和与酶 (CAT) 的相互作用.
研究的目的:
- 研究由HHBNs引起的氧化应激生物标志物和细胞损伤.
- 探索HHBNs和催化酶 (CAT) 之间的结合相互作用.
- 阐明新出现的HHBN DBPs的有毒机制.
主要方法:
- 在HHBN处理的细胞中检查氧化应激和损伤生物标志物.
- 使用生物化学测试,探索3,5-dibromo-2-hydroxybenzonitrile和CAT之间的结合相互作用.
- 在HHBN细胞毒性和CAT抑制功效之间的相关性分析.
主要成果:
- 暴露于3,5-dibromo-2-hydroxybenzonitrile干扰了抗氧化系统 (CAT,超氧化失调酶,谷氨) 并增加了反应性氧物种.
- 氧化应激导致DNA损伤和随后的细胞亡.
- 3,5-dibromo-2-hydroxybenzonitrile与CAT的结合改变了它的结构和活性,主要是通过范德瓦尔斯力和结合.
结论:
- HHBNs通过氧化应激,DNA损伤和亡诱导细胞毒性,部分通过CAT抑制.
- 在HHBN细胞毒性和CAT抑制作用之间存在正相关性.
- 未确定的化HBNs可能具有高细胞毒性,需要进一步调查.
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