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抗微生物耐药性Enterobacter物种的临床分离物可以在人类巨细胞中持续存在,没有复制和明显的细胞毒性

Georgiana Parau¹, Hannah J Parks¹, Amy J G Anderson¹

¹Infection Biology Group, Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, Belfast, BT9 7BL, United Kingdom.

The Journal of infectious diseases

|March 4, 2025

概括

肠杆菌细菌可以在人体巨细胞内长期生存而不复制,可能导致患者的持续感染和复发. 这项研究揭示了它们的细胞内生存机制.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

肠杆菌物种是机会性,多药耐药的格拉姆阴性细菌,导致全球显著的发病率和死亡率.
关于Enterobacter spp.感染生物学和细胞内行为的知识有限.

研究的目的:

为了研究人类巨细胞内Enterobacter临床分离物的细胞内贩运.
为了确定宿主巨对细胞内Enterobacter感染的反应.

主要方法:

11个临床Enterobacter分离体的化细胞被原发性人类巨细胞所分离.
同焦点光显微镜用于追踪细胞内细菌贩运.
细菌计数和光稀释以评估复制;乳酸脱酶释放和炎症标志物裂变以测量细胞毒性.

主要成果:

肠杆菌分离物在巨细胞内在细胞内存活了多达44小时,没有复制.
细胞内存活率与胆固醇耐药性,LPS修饰或Galleria mellonella致病性没有相关性.
观察到较低的巨细胞毒性,关键炎症标志物的分裂最小.

结论:

肠杆菌 spp. 进入细菌 spp. 临床分离物可以在巨细胞中细胞内持续存在而不复制,造成最小的宿主细胞损伤.
这种细胞内持久性可能会导致长期感染和复发,患者无法清除细菌.

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