调料微稀释和Etest®方法的比较,用于对Candida auris中的安福特素B的敏感性测试
Domenica G De Luca1,2, Xiao Rui Li1, David C Alexander2,3
1National Microbiology Laboratory Branch, Public Health Agency of Canada, Winnipeg, MB, R3E 3R2, Canada.
Medical mycology
|March 4, 2025
概括
使用Etest和汁微稀释方法对Candida auris的抗真菌敏感性测试显示了显著的差异. 苏微稀释可能更可靠地检测Candida auris.中的安福特素B耐药性.
科学领域:
- 医学真菌学 医学真菌学
- 抗微生物耐药性 抗微生物耐药性
- 临床微生物学 临床微生物学
背景情况:
- 氨酸B对于治疗多抗药性Candida auris感染至关重要.
- 准确的抗真菌敏感性测试 (AST) 对于指导治疗决策至关重要.
- 方法上的变化可能会影响Candida auris.的AST结果.
研究的目的:
- 为了比较Etest和临床和实验室标准研究所 (CLSI) 微稀释 (BMD) 方法对Candida auris.中的安福特素B AST.
- 为了评估两种方法之间的电阻解释的差异.
主要方法:
- 测试了60种临床Candida auris分离物,来自四大类.
- 使用Etest和CLSI BMD参考方法进行了安福特里辛B AST.
- 分析的最小抑制度 (MIC) 和分类一致性.
主要成果:
- 在Etest和BMD方法之间观察到MIC的显著差异 (P < .0001).
- 在阻力解释中发现了很高的差异率,包括33.3%的非常重大错误率.
- 方法之间的绝对一致率为88.3%.
结论:
- 测试和CLSI BMD方法在Candida auris.中对安福特素B敏感性的结果显著不同.
- 苏微稀释可能提供一种更保守和可靠的方法来检测安二B耐药性.
- 对AST方法的标准化对于准确的临床治疗Candida auris感染至关重要.
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