相关实验视频

Updated: May 24, 2025

Visualization of Organelles In Situ by Cryo-STEM Tomography
08:37

Visualization of Organelles In Situ by Cryo-STEM Tomography

Published on: June 23, 2023

2.0K

细胞生物学的冷-STEM断层扫描使用厚厚的拉美拉

Kazuhiro Aoyama1,2, Hiroko Takazaki3, Misaki Arie3

  • 1NanoPort Japan Application Laboratory, Thermo Fisher Scientific Shinagawa Seaside West Tower 1F, 4-12-2 HigashiSinagawa, Shinagawa-ku, Tokyo 140-0002 Japan, TEL: 03-3740-0970.

Microscopy (Oxford, England)
|March 4, 2025
PubMed
概括

扫描传输电子显微镜 (STEM) 断层扫描克服了用于细胞生物学的电子断层扫描 (ET) 的厚度限制. 这种技术可以对更厚的生物标本进行详细的3D重建,从而推进结构研究.

关键词:
细胞结构 细胞结构冰式断层扫描 (Cryo-Tomography) 是一个非常简单的方法.聚焦的离子束.拉梅拉拉梅拉拉梅拉拉拉拉拉拉拉拉拉拉拉拉这是一个声音.一个厚厚的标本.

更多相关视频

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography07:00

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

Published on: August 5, 2021

3.2K
A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography08:47

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

Published on: March 15, 2021

3.8K

相关概念视频

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.3K
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.2K