脊椎动物在出生后表现出线粒体和核基因的协调高表达
1Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel.
Genome research
|March 4, 2025
概括
线粒体-核基因表达 (CMNGE) 的协调调节在脊椎动物出生后急剧增加,表明一种保存的代谢开关. 像PPARGC1A和CEBPB这样的关键监管机构被提升,突出了他们在这个重要的发展过渡中的作用.
科学领域:
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
- 代谢过程中的代谢.
背景情况:
- 线粒体和核因素的相互作用对生命至关重要.
- 线粒体-核基因表达 (CMNGE) 的协调调节至关重要,但在各种生理条件和物种中了解得很少.
- 在脊椎动物发育过程中,CMNGE的保存在很大程度上是未知的.
研究的目的:
- 调查CMNGE在脊椎动物发育过程中是保存和重要的假设.
- 分析脊椎动物在产前和产后发育期间的CMNGE模式.
- 在过渡到新生儿期间识别CMNGE的潜在调节者.
主要方法:
- 分析了1400多个RNA-seq实验,涵盖了脊椎动物进化中的产前发育到成年期.
- 对哺乳动物的基因表达数据进行比较分析,包括Gallus gallus,Anolis carolinensis,Danio rerio,Xenopus tropicalis,Caenorhabditis elegans和Drosophila melanogaster.
- 检查已知的线粒体基因表达调节者的表达模式.
主要成果:
- 在哺乳动物,鸟类和爬行动物的多种组织中,在出生/化后观察到CMNGE的保存,急剧升高,包括氧化化 (OXPHOS) 和线粒体核糖体基因.
- 这种产后CMNGE增加与TCA循环酶表达升高和低氧反应基因减少有关,这表明代谢切换.
- 在出生/化后发现PPARGC1A和CEPB的表达持续升高,将其确定为候选CMNGE调节剂.
- 相比之下,Xenopus tropicalis和Drosophila melanogaster在化前显示CMNGE升高,与肌肉活动有关,这种模式在哺乳动物和中不存在,表明进化损失.
结论:
- 出生后受调控的CMNGE代表了一种关键的代谢切换器,在脊椎动物和强大的选择性压力下保存.
- 在过渡到新生儿期间,PPARGC1A和CEPB被确定为CMNGE的关键候选监管者.
- 这项研究揭示了CMNGE模式的进化分歧,在陆地脊椎动物中失去了古老的化前模式.
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