Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Diagnostic challenges and Gram-negative pathogen dominance in early- and late-onset neonatal infection in Manila, Philippines.

Nature communications·2026
Same author

Mortality and Length of Stay Associated With Community-Acquired Escherichia coli and Staphylococcus aureus Bloodstream Infections: An Observational Study in 9 Low- and Middle-Income Countries.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2026
Same author

Acrobatics for Antibiotics: Exploring circus-based engagement on community practices surrounding antimicrobial resistance in Cambodia.

Wellcome open research·2026
Same author

Predicting referral need for febrile children in low-resource community settings in South and Southeast Asia.

Nature medicine·2026
Same author

Unlocking CRISPR-Cas9 editing for widely diverse Dictyostelid species.

Molecular systems biology·2026
Same author

Circus arts shine a spotlight on antimicrobial resistance in Cambodia.

International health·2026
Same journal

Complete sequencing of medaka genomes reveals the architecture of centromeric satellites, giant mobile elements, and sex chromosomes.

Genome research·2026
Same journal

Convergence and conflict among telomere specialized transposons across 60 million years of Drosophilid evolution.

Genome research·2026
Same journal

A unified analysis of cell type- and trajectory-associated pathways in single-cell data using Phoenix.

Genome research·2026
Same journal

Resf1 is required for proper placental development and configuration of trophoblast cell-specific heterochromatin.

Genome research·2026
Same journal

Telomere-driven replicative crisis is driven by large-scale changes in genomic architecture.

Genome research·2026
Same journal

Spatially informed reference-free cell-type deconvolution for spatial transcriptomics with SpatialCD.

Genome research·2026
查看所有相关文章

相关实验视频

Updated: May 24, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

13.5K

优化纳米孔适应性采样用于复杂样品中的肺炎球菌血清型监测,使用基于图形的GNASTy算法.

Samuel T Horsfield1,2, Basil C T Fok3, Yuhan Fu3

  • 1MRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology, Imperial College London, London W12 0BZ, United Kingdom; shorsfield@ebi.ac.uk.

Genome research
|March 4, 2025
PubMed
概括
此摘要是机器生成的。

针对囊生物合成位点 (CBL) 的纳米孔适应性采样 (NAS) 可实现精确的Streptococcus pneumoniae血清定型,即使具有多个菌株. 一个新的泛基因组图算法,GNASTy,增强了新型血清型检测.

更多相关视频

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings
10:26

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

5.0K
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

8.7K

相关实验视频

Last Updated: May 24, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

13.5K
Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings
10:26

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

5.0K
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

8.7K

科学领域:

  • 微生物学和基因组学 微生物学和基因组学
  • 疫苗开发和流行病学

背景情况:

  • 对于评估疫苗有效性而言,对肺炎 estreptococcus (肺炎球菌) 的血清型监测至关重要.
  • 目前的血清型定型方法难以检测多个肺炎球菌菌株 (共携) 和识别新血清型.

研究的目的:

  • 开发一种可扩展,便携和准确的方法,从复杂的样本中直接进行肺炎球菌血型鉴定.
  • 克服现有方法在检测共同携带和新型血清型方面的局限性.

主要方法:

  • 利用纳米孔适应性采样 (NAS) 进行实时,在测序器上的DNA丰富.
  • 针对肺炎球菌囊生物合成位点 (CBL) 进行特定的血清型识别.
  • 开发和评估了一种新的泛基因组图算法,GNASTy,用于增强NAS灵敏度.

主要成果:

  • 针对CBL的NAS显示出S. pneumoniae检测的高特异性和灵敏性.
  • NAS显著改善了CBL覆盖率,并准确量化了共运样本中的血清型流行率.
  • 在检测目标数据库中缺少的血清型中,GNASTy的表现优于基于线性对齐的NAS.

结论:

  • 开发的NAS和GNASTy方法为肺炎球菌血清型监测提供了改进的方法.
  • 这些方法是快速的,准确的,并且可用于低资源的环境,有助于新型血清型的发现.
  • 使用GNASTy的向丰富优化了复杂临床样本中的血清型监测.