在口腔状细胞癌中对HOXA10-AS进行自然反意义转录介导调节
Kanaka Sai Ram Padam1, Satyajit Dey Pereira1, Naveena A N Kumar2
1Department of Cell and Molecular Biology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, India.
概括
在口腔癌中,HOXA10-AS通过反感性机制调节HOXA10的表达. 这种表观遗传调节,涉及非甲基化促进体标记,影响细胞进展和转移.
科学领域:
- 在瘤学瘤学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 已知HOXA10-AS和HOXA10在癌症中的致癌作用.
- 它们在口腔状细胞癌 (OSCC) 中的表观遗传调节和反感介导控制仍然不清楚.
研究的目的:
- 研究HOXA10的表观遗传作用和HOXA10-AS在OSCC进展中的反感调节.
- 在OSCC中阐明HOXA10-AS和HOXA10的调节机制.
主要方法:
- 在使用RT-qPCR的OSCC标本中分析HOXA10-AS和HOXA10表达.
- 甲基捕获测序,光酶测定和ChIP-qPCR用于研究表观遗传修饰和促进剂活性.
- 通过siRNA介导的HOXA10-AS的淘汰,以评估其对细胞增殖,循环和迁移的功能影响.
主要成果:
- 在淋巴结阳性OSCC中,HOXA10-AS和HOXA10过度表达.
- HOXA10的远端促进体是通过非甲基化标记和基因素修饰来表观遗传调节的.
- 降低HOXA10-AS降低了HOXA10的表达,抑制了细胞周期的进展,并降低了上皮细胞到介质酶细胞的过渡.
结论:
- HOXA10-AS通过自然的反意义机制来调节HOXA10的表达.
- 通过HOXA10的远端促进体中的非甲基化标记进行表观遗传调节对于OSCC的进展至关重要.
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