相关实验视频
Updated: May 24, 2025

14:57
Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
9.5K
p53以p21独立的方式调节DREAM复杂介导的抑制
Ritu Agrawal1, Sagar Sengupta2,3
1Biotechnology Research and Innovation Council-National Institute of Immunology (BRIC-NII), Aruna Asaf Ali Marg, New Delhi, 110067, India.
The EMBO journal
|March 4, 2025
概括
瘤抑制剂p53通过一种新的非转录机制直接抑制DREAM目标基因. 这一发现揭示了对p53的新见解.
科学领域:
- 细胞生物学 细胞生物学
- 分子瘤学分子瘤学
- 基因调节 基因调节
背景情况:
- 梦想复合体对于细胞周期控制和DNA修复至关重要,维持基因组的稳定性.
- 通常,p53通过p53-p21-Rb通路抑制基因,但对DREAM目标的直接调节尚未被探索.
研究的目的:
- 调查p53直接调节DREAM目标的潜力,独立于其转录活动.
- 阐明DREAM目标的p53中介镇压机制.
主要方法:
- 在具有不同p53和p21状态的细胞中分析DREAM目标基因去抑制.
- 利用野生类型和缺乏交换激活的p53突变来评估抑制机制.
- 执行Re-ChIP测试以确认p53和E2F4的共同招募到目标发起人.
主要成果:
- 比起单独的p21损失,p53损失导致了DREAM目标的更大去压力.
- 野生类型和缺乏交换的p53都抑制了DREAM目标,这表明了一个非正规的机制.
- p53变体与p130/p107结合,与癌症相关的突变体封存E2F4,破坏DREAM复合体的功能.
- 重新ChIP证实直接p53和E2F4绑定到DREAM的目标促进者.
结论:
- 通过一种独立于交换激活的机制,p53直接抑制DREAM目标基因.
- 这种新的抑制途径扩大了对p53瘤抑制功能的理解.
- 准DREAM复合体为癌症治疗提供了潜在的新途径.
相关概念视频
Abnormal Proliferation
4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
Negative Regulator Molecules
35.1K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.1K
DNA Damage can Stall the Cell Cycle
9.0K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.0K
Interactions Between Signaling Pathways
6.2K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.2K
Inhibition of Cdk Activity
4.6K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.6K
Covalently Linked Protein Regulators
6.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
6.8K

