在组装后的等离子体放大用于增加在E.E.中的转换产量. 大肠杆菌和S. coli和S. 类植物

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Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly
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Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly

Published on: February 9, 2015

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Thomas Fryer1,2, Darian S Wolff1,2, Max D Overath1

  • 1Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads 239, Lyngby, Hovedstaden DK 2800, Denmark.

Chem & bio engineering
|March 5, 2025
PubMed
概括

研究人员开发了一种简化方法,使用滚动圆放大 (RCA) 和内核酶来提高细胞转化DNA产量. 这提高了细菌和酵母中的DNA工程效率,有助于遗传研究和合成生物学应用.

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Bacterial Transformation01:33

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...