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简单的EWAS:一个灵活和用户友好的R包,用于全表观基因组关联研究

Yuting Wang¹, Meijie Jiang¹, Siyuan Niu¹

¹Department of Occupational and Environmental Health Sciences, School of Public Health, Peking University, Beijing, 100191, China.

Bioinformatics advances

|March 5, 2025

概括

easyEWAS是一个用户友好的R包,简化了使用Illumina甲基化Beadchips的表观基因组广泛关联研究 (EWAS). 它简化了分析和解释,使得DNA甲基化研究在公共卫生和临床环境中更容易获得.

科学领域:

基因组学和生物信息学
表观遗传学和DNA甲基化分析

背景情况:

高通量测序,特别是Illumina DNA甲基化珠芯片,已经加速了全表观基因组关联研究 (EWAS).
现有的EWAS工具往往很复杂,这阻碍了研究人员和临床医生具有有限的生物信息学专业知识的可访问性.
这种复杂性使研究设计,下游分析和DNA甲基化数据的解释变得复杂.

研究的目的:

为DNA甲基化研究开发一个灵活和用户友好的R包,easyEWAS.
为了简化执行EWAS的过程,使用Illumina DNA甲基化珠芯片.
为了促进下游分析,结果解释和验证在临床和公共卫生研究.

主要方法:

将最先进的EWAS分析方法集成到一个单一的R包中.
包括差异甲基化位置和区域分析 (DMRcate) 的统计方法.
整合了全面的功能注释,结果可视化和基于引导的内部验证.

主要成果:

易易EWAS为使用Illumina甲基化珠芯片的EWAS提供了一种简化的工作流.
该包支持差异甲基化分析,功能注释和可视化.
简化EWAS用于喘研究的实用性,提高公共卫生和临床应用的可访问性.

结论:

简单的EWAS显著简化和简化基于DNA甲基化的研究.
该方案提高了临床医生和公共卫生专业人员对EWAS的可访问性.
easyEWAS在公共卫生和临床环境中有效推进DNA甲基化研究.

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